猪瘟病毒E2蛋白B细胞识别基序的筛选  

Screening for B cell liner epitope on E2 protein of classical swine fever virus

作　　者：王爱萍[1] 马丽平[1] 李姣[1] 邓瑞广[2] 邢广绪[2] 郝慧芳[2] 

机构地区：[1]郑州大学生物工程系,河南郑州450001 [2]河南省动物免疫学重点实验室,河南郑州450002

出　　处：《中国兽医科学》2008年第12期1045-1049,共5页Chinese Veterinary Science

基　　金：国家自然科学基金项目(30471287)

摘　　要：根据猪瘟病毒(classical swine fever virus,CSFV)E2蛋白的序列设计并人工合成18条重叠多肽,与载体蛋白BSA偶联后,利用抗CSFV E2蛋白单克隆抗体SWmAb8筛选该蛋白上的B细胞表位;同时用12肽噬菌体随机肽库淘选E2单抗识别多肽序列。结果显示,多肽序列VSPTTLRTEV能被单抗SWmAb8识别;含SPTxLR基序的噬菌体能被单抗SWmAb8结合,且能被病毒抗原竞争性抑制。证实SPTxLR基序很可能是E2蛋白的线性表位之一,可诱导机体产生中和抗体。Eighteen polypeptides designed and synthesized according to the amino acid sequence of E2 protein of classic swine fever virus（CSFV） were conjugated with BSA. Monoclonal antibody SWmAb8 was used to select the epitope and was also used in biopanning in phage displayed random 12-peptide library. The results showed that the polypeptide VSPTTLRTEV could be recognized by SWmAb8,and the motif of SPTxLR could bind to SWmAb8, and could be competitively inhibited by virus particles in a dose dependent manner. The results indicated that the motif SPTxLR might be one of the liner B cell epitopes inducing neutralizing antibody in organism.

关 键 词：猪瘟病毒 E2蛋白 B细胞表位 重叠多肽扫描 噬菌体随机肽库 

分 类 号：S852.659.6[农业科学—基础兽医学]