深低温冷冻异体肌腱移植的组织学和生物力学研究  

Histological and biomechanical study of deep-frozen allogenetic tendons

作　　者：徐光辉[1] 孙康[2] 徐强[1] 盖鹏宙[1] 孙洪亮[1] 

机构地区：[1]青岛大学医学院附属烟台毓璜顶医院骨科,山东烟台264000 [2]青岛大学医学院附属医院关节外科,山东青岛266003

出　　处：《中国矫形外科杂志》2008年第24期1883-1886,共4页Orthopedic Journal of China

摘　　要：[目的]探讨深低温冷冻同种异体肌腱替代自体肌腱进行移植治疗肌腱缺损的可行性及移植后异体肌腱的形态学和生物力学性能的动态变化。[方法]采用兔跟腱缺损修复模型,经深低温冷冻的同种异体跟腱为实验组,自体跟腱移植为对照组,分别在术前及术后2、4、8周时对实验组同种异体跟腱及对照组自体跟腱进行大体观察、组织学检查和生物力学测试。[结果]同种异体跟腱与自体跟腱在移植前和移植后2、4、8周时其大体观察、组织学检查和生物力学测试等各项指标均无明显差异,显示同种异体跟腱和自体跟腱有相类似的结构及愈合过程。另外,同种异体跟腱在移植后其力学性能(除衰竭应变外)均比移植前明显降低。随时间推进有上升的趋势,但8周时仍明显低于移植前水平。[结论]深低温冷冻同种异体肌腱可替代自体肌腱应用于移植修复肌腱缺损。同时,由于移植后肌腱力学性能明显下降,需给予适当保护,防止过度牵拉而导致衰竭。[Objective] To explore the allograft, as a substitute for autograft, whether can be used to repair the defect of the tendon and restore the stability of joint and the dynamic biomechanical changes of allogTaft after transplantation. [Method] Deep-frozen allogeneic achilles tendons of rabbits were used as treatment group, and autogenous ones as control. The achilles tendon defects of back limbs were repaired by allograft and autograft separately. The macroscopic, microscopic observation and biome-chanical test were performed on both of them before transplantation and in 2, 4 and 8 weeks after transplantation. [ Result] It was showed that there were no differences in macroscopy, microscopy and mechanical strength between allograft and autograft both before and after transplantation. They may have experienced the similar healing course. The mechanical strength of allograft （ except failure strain） was reduced significantly after transplantation. But it had a rising trend with time passing, although it was fairly low in 8 weeks compared with the normal such as maximum load. [ Conclusion] The results demonstrate that the deep-frozen allograft can substitute for autograft in repairing the tendon defect, and because of the weakness of allograft after transplantation, it needs appropriate protection to prevent failure by excessive strain in early stage.

关 键 词：肌腱 同种异体移植 深低温冷冻 组织学 生物力学 

分 类 号：R687.2[医药卫生—骨科学] R318.01[医药卫生—外科学] R318.52[医药卫生—临床医学]