体外培养的肾母细胞瘤细胞株与正常胚肾细胞株差异蛋白的表达  

Differential protein expression between Wilms tumor and embryonic kidney cells

作　　者：王家祥[1] 贾佳[1] 刘秋亮[1] 安乾[1] 余捷凯[1] 郑树[1] 

机构地区：[1]郑州大学第一附属医院小儿外科,450052

出　　处：《中华小儿外科杂志》2008年第12期752-756,共5页Chinese Journal of Pediatric Surgery

基　　金：国家科学自然基金项目资助（编号：30772273）

摘　　要：目的运用SELDI蛋白质芯片技术分析体外培养的肾母细胞瘤细胞株（G401）与正常胚肾细胞株（CCC-HEK-1）蛋白质表达差异。方法在体外培养G401和CCC-HEK-1细胞株，收获细胞和培养液，将细胞用细胞裂解液裂解后，采用SELDI—TOF-MS技术用WCX2芯片检测（2-401和CCC-HEK-1的蛋白质谱差异。结果体外培养的肾母细胞瘤细胞株与正常胚肾细胞株的蛋白质存在差异表达，WCX2芯片共发现差异峰19个，与CCC-HEK～1细胞相比，其中12个在肾母细胞瘤细胞株中高表达，7个差异蛋白在肾母细胞瘤细胞株中低表达。G401细胞裂解液与培养液的蛋白质表达差异峰19个，14个差异蛋白在G401细胞裂解液中高表达，5个在G401细胞裂解液中低表达；G401细胞裂解液与培养液的比较相似蛋白质为3个。结论SELDI蛋白芯片技术检测肾母细胞瘤细胞株与正常胚肾细胞株蛋白质的差异表达方法简便，灵敏度高，重复性好。Objective To investigate the differential proteins expression between Wilms＇ tumor cell line G401 and embryonic kidney cell line CCC-HEK-1 cultured in vitro using surface enhanced laser desorption/ionization （SELDI） protein array technology. Methods The in vitro cultured Wilms＇ tumor cell line G401 and embryonic kidney cell line CCC-HEK-1 along with their culture solution were harvested and then split by cell lysate solution. The protein mass spectra of the G401 and CCC-HEK-1 cell were detected by WCX2 based on SELDI protein array technology. Results Significant difference of protein expression existed in the two cell lines. A total of 19 differential peaks were found by WCX2 array. Among the differentially expressed proteins, twelve were highly expressed, while the other 7 were lowly expressed in the G401 cells. A total of 19 differential peaks were found in culture solution and lysate of the G401, in which, 14 were highly expressed, while 5 were lowly expressed in the lysate of the G401. A total of 3 similar peaks were found in culture solution and lysate of the G401. Conclusions The SELDI Protein Chip technology can be served as a desired method in detecting the biological markers in early period of Wilms＇ tumor. The biological tags of tissue-specific proteins we found have potential applying values in the early diagnosis of Wiling tumor.

关 键 词：肾母细胞瘤 蛋白质芯片 患者 临床治疗 

分 类 号：R686[医药卫生—骨科学]