转基因植物检测技术研究进展  

Development of the Testing Technology for Transgenic Plants

作　　者：王瑾[1,2] 唐益苗[3] 赵昌平[3] 叶兴国[1] 

机构地区：[1]中国农业科学院作物科学研究所,北京100081 [2]东华大学化学化工与生物工程学院,上海201620 [3]北京市农林科学院北京杂交小麦研究中心,北京100089

出　　处：《科技导报》2008年第23期88-93,共6页Science & Technology Review

基　　金：中国高技术研究发展计划(863计划)项目(2007AA10Z129);中央级公益性科研院所基本科研业务费专项;北京市科技计划项目(Z07070501770702)

摘　　要：随着植物基因工程技术的发展,转基因植物的研究和开发取得了令人瞩目的成绩,已培育了一批抗虫、抗病、抗除草剂和高产优质的转基因农作物新品种。与此同时,转基因植物检测技术不断丰富和完善,促进了转基因植物科学鉴定和评价,以及转基因植物商业化种植。目前报道的转基因植物检测方法主要有3类,一是在整合水平上进行的检测,包括PCR检测、Southern blot检测和染色体原位杂交检测等;二是在转录水平上进行的检测,包括RT-PCR检测、Northern blot杂交检测等;三是在表达水平上进行的检测,包括组织化学染色检测、荧光蛋白检测、Western blot检测、ELISA检测、叶片退绿检测和叶片涂抹除草剂检测等。With the rapid development of plant genetic engineering, remarkable achievements have been made on the research and commercialization of transgenic plants. A number of genetic modified crops have been developed with dramatic improvement of characteristics, such as insect resistance, disease resistance, herbicide resistance, high yield and good quality. At the same time, detection methods of transgenic plants have been perfected and the ever increasing creations have promoted a further development of evaluation techniques and applications of transgenic plants. Theidentification techniques for transgenic plants can be classified into three main types, those in integration levels such as PCR, southern blot and chromosome in situ hybridization, those in transcription levels such as RT-PCR and northern blot, and those in expression levels such as GUS histochemical staining, green fluorescent protein observation, western blot, ELISA assay, leaf bleach assay, leaf painting and spraying or dropping with antibiotics.

关 键 词：转基因植物 整合 转录 表达 分子检测 

分 类 号：Q943.2[生物学—植物学]