一株高效高温菌的筛选初步鉴定和性质研究  

Screening,identification and properties of a strain of thermophilic bacterium with strong degradation ability

作　　者：秦蓉[1] 李秀艳[1] 李华芝 韩波波[1] 黄民生[1] 

机构地区：[1]华东师范大学环境科学系,上海200062 [2]上海新东京(集团)有限公司,上海200232

出　　处：《华东师范大学学报（自然科学版）》2008年第6期82-87,102,共7页Journal of East China Normal University(Natural Science)

基　　金：上海市科委重大项目(06dz12006);国家高科技研究发展863计划专项基金(2006AA06Z331);上海市科技成果转化促进会和上海市教育发展基金会2006年红双喜《联盟计划》

摘　　要：从高温堆肥物料中通过平板培养法分离、纯化并筛选出一株高效高温菌YB16.YB16具有同时分解淀粉、蛋白质、油脂和纤维素等大分子有机物的能力.经鉴定YB16为革兰氏阳性,杆状,有芽孢,好氧,G＋C mol%为50.66%,初步鉴定YB16属于芽孢杆菌属.pH生长范围为6.0～9.0,最适生长温度为65℃左右.A strain of thermophilic bacterium with strong degradation ability named YB16 was plate screened from thermophilic compost materials. The selected strain can decompose organic macromolecules such as starch, protein, lipid and cellulose simultaneously. YB16 was aerobic, Gram positive, rod, and formed spore and capsule. The results of DNA UV absorbency showed that G＋ C mol% of YB16 was about 50.66%. It was considered to be a variety of Bacillus sp. YB 16 grew well at pH 6.0-9.0. The optimal growth temperature was about 65 ℃.

关 键 词：高温菌 鉴定 芽孢杆菌 降解能力 G+Cmol% 

分 类 号：Q948[生物学—植物学]