纤毛虫胞质微管及大核的FLUTAX-EB荧光标记方法  

An improved FLUTAX-EB fluorenscent labeling method for visualizing microtubule and nucleolus of ciliate

作　　者：李艺松[1] 刘兰侠[1] 史磊[1] 顾福康[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《华东师范大学学报（自然科学版）》2008年第6期133-136,共4页Journal of East China Normal University(Natural Science)

基　　金：国家自然科学基金(30470201)

摘　　要：以游仆虫、尖毛虫和尾柱虫三种腹毛目纤毛虫为材料,在应用FLUTAX方法显示纤毛虫细胞微管结构的标本制备过程中,增加了EB染色的歩骤,使标记纤毛虫细胞皮层微管的同时也显示了细胞核的结构.并提出了实验中对EB浓度及渗透时间等需注意的方面.An employment of EB in FLUTAX fluorescent labeling porcedure was proposed, with the aim of visualizing both microtubular pattern and nucleolus of three species of ciliate including Euplotes woodruffi, Oxytricha sp. and Urostyla sp.. The concentration of EB, as welt as the infiltrating time was also discussed.

关 键 词：纤毛虫 皮层 FLUTAX-EB荧光标记 方法改进 

分 类 号：Q948[生物学—植物学]