壳聚糖支架材料的制备、表面修饰及细胞粘附性能的研究  

Cell Adhesion Behavior,Fabrication and Surface-Modification of Chitosan Scaffolds

作　　者：文鹏飞[1] 宋克东[1] 刘天庆[1] 朱艳霞[1] 

机构地区：[1]大连理工大学干细胞与组织工程研究室,辽宁大连116024

出　　处：《高校化学工程学报》2008年第6期992-998,共7页Journal of Chemical Engineering of Chinese Universities

基　　金：国家自然科学基金(30670525);校青年教师培养基金项目(893228);大连市科学技术基金(2005E11SF068)项目资助

摘　　要：通过冷冻干燥法进行壳聚糖(Chitosan)多孔支架材料的制备,使用I型明胶对所制得的支架材料进行表面修饰,用于生物医用可降解复合材料的仿生构建。对所制备的支架材料分别进行密度、孔隙率和降解率等物理性质测定,并使用新西兰兔来源的骨髓间充质干细胞(Bone marrow mesenchymal stem cells,BMSCs)检验支架材料的细胞粘附和生物相容性能。以未经表面处理的该种材料作为对照组,对MSCs-Chitosan复合物分别进行扫描电镜和Hoechst染色等,检测MSCs(间充质干细胞)在支架材料表面的生长、粘附和分布形态。结果显示两种壳聚糖浓度制备的多孔支架材料的空隙率分别为(86.85±2.42)%和(71.50±3.29)%,孔径为90～130μm,MSCs在经明胶表面修饰的材料表面表现出良好的粘附、伸展性能。此外,本研究还对1-乙基-(3-二甲基氨基丙基)碳酰二亚胺(1-ethyl-(3-dimethylanimopropyl)carbodiimide,EDC)交联剂对壳聚糖基支架性能的影响进行了初步研究。研究表明,通过明胶修饰构建的仿生明胶/壳聚糖人工基质材料,具有良好的生物相容性能,作为一种理想的组织工程用支架材料,适于细胞在其上的粘附与增殖。The chitosan scaffolds were fabricated by lyophilization technique, and the prepared scaffolds were surface-modified by the gelatin to biomimetically construct a degradable composite for biomedical application. The density, porosity and degradation of the fabricated and modified scaffolds were determined; the cell adhesion behavior and biocompatibility of the treated scaffolds were detected by inoculation of the bone marrow mesenchymal stem ceils （BMSCs） derived from New Zealand rabbits, and the MSCs were inoculated onto the untreated scaffold too, which was used as the control sample group. The MSCs-chitosan composites were stained with Hoechst method and observed under fluorescence microscopes and scanning electron microscope （SEM） to study the cell growth, adhesion and distribution of the MSCs. The results show that the porosities of the scaffolds prepared with two different chitosan concentrations are （68.85±242）% and （71.50±3.29）%, respectively; the pore size is 90-130 μm, and the MSCs inoculated onto the treated surfaces present good adhesion and spreading ability. Besides, the effect of the crosslinking agent 1-ethyl- （3-dimethylanimopropyl） carbodiimide （EDC） on the performance of the scaffolds was initially investigated in this study. It can be concluded that the gelatin surface-modified biomimetic scaffolds have excellent biocompatibility and are appropriate for cell adhesion and proliferation. Therefore, it would be a promising scaffold for tissue engineering application.

关 键 词：壳聚糖 多孔支架材料 表面修饰 骨髓间充质干细胞 细胞粘附 

分 类 号：R318.08[医药卫生—生物医学工程]