转基因抗虫玉米Bt毒蛋白的时空表达分析  

Studies on the Temporal and Spatial Expressions of Bt Toxin Protein of Bt Transgenic Maize

作　　者：姜志磊[1] 刘德璞[1] 李晓辉[1] 孔祥梅[1] 李葱葱[1] 李飞武[1] 孙传波[1] 袁英[1] 

机构地区：[1]吉林省农业科学院生物技术研究中心,长春130033

出　　处：《吉林农业科学》2008年第6期35-37,共3页Journal of　Jilin Agricultural Sciences

基　　金：国家转基因中试及产业化基地专项(JY03-17-1)资助

摘　　要：对2个转Bt基因玉米抗虫系株系进行Bt毒蛋白表达量的分析测定。结果显示,转基因株系中的毒蛋白可以在其后代稳定的遗传和表达,毒蛋白的表达为组成性表达,在玉米的各个发育时期和植株的各个部位均有表达,但其表达的量有显著差异,毒蛋白表达量随着植株的生长而减少,比较植株的各个器官,叶片毒蛋白含量最高,茎次之,根、种子和花丝中的毒蛋白含量较少。The content of Bt （Bacillus thuringiensis Berliner） toxin protein of two Bt transgenic maize was tested. The results showed that Bt toxin protein was detected in all the test organs and phase of the transgenic maize, but its contents varied significantly among different organs in different stages. The expression of Bt toxin protein in Bt transgenic maize decreased with advance of plant development. Among different organs, the highest Bt toxin protein content in the leaves, less in stem, and the least in root, seed and faliment.

关 键 词：玉米 BT 转基因 

分 类 号：S513[农业科学—作物学]