马鹿精子膜蛋白提取及理化性质分析  

Extraction of Sperm Membrane Proteins from Cervus elaphus and Its Physicochemical Properties

作　　者：柯冬蕾[1] 李和平[2] 徐境羚[1] 

机构地区：[1]东北林业大学,哈尔滨150040 [2]东北林业大学野生动植物资源学院

出　　处：《东北林业大学学报》2008年第11期83-85,共3页Journal of Northeast Forestry University

基　　金：黑龙江省科技攻关资助项目(GC02B514);哈尔滨市自然科学基金资助项目(2003AFXXJ016);东北林业大学优秀青年教师创新资助项目

摘　　要：采用非离子型去污剂(NP-40)裂解法提取马鹿精子膜蛋白,确定出马鹿精子膜蛋白的最佳分离胶质量分数为12%。通过对膜蛋白的性质分析,发现马鹿精子膜蛋白是一组具弱酸性等电点的低相对分子质量糖蛋白,相对分子质量为21.7～98.4kDa,等电点为pI4～9。An experiment was conducted to extract sperm membrane proteins from Cervus elaphus by lysis method with non-ionic decontaminant （NP-40）. The optimal concentration of separation gel was determined as 12%. Sperm membrane proteins in C. elaphus were a group of weak acid glyeoproteins with low molecular weight according to the physieoehemical properties of the sperm membrane proteins, and their molecular weights ranged from 21.7 kDa to 98.4 kDa and isoeleetric points were between 4 and 9.

关 键 词：马鹿 精子膜蛋白 理化性质 

分 类 号：TQ333.2[化学工程—橡胶工业] Q78[生物学—分子生物学]