高效液相色谱法测定丹参提取物中3种水溶性成分的含量  

Determination of 3 Water-soluble Components in the Extract from Salvia miltiorrhiza Bge.by HPLC

作　　者：典灵辉[1] 崔燎[1] 吴铁[1] 

机构地区：[1]广东医学院,广东东莞523808

出　　处：《时珍国医国药》2008年第12期2861-2863,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30672470)

摘　　要：目的建立高效液相色谱（HPLC）法测定丹参提取物中水溶性成分丹参素、原儿茶醛和丹酚酸B含量的方法。方法高效液相色谱梯度洗脱法。色谱柱为Hypersil ODSC18色谱柱（250mm×4．6mm，5μm）；流动相为1％冰醋酸溶液（A）和甲醇（B）梯度洗脱；检测波长为281nm；流速为1．0ml·min^-1；柱温25℃。结果丹参素、原儿茶醛和丹酚酸B线性范围分别在0．26～2．08μg，0．13～1．04μg，0．45～3．60μg内线性关系良好（r≥0．9996）；加样回收率分别为97．76％，99．56％，99．68％，RSD〈3．4％。结论该方法操作简便、灵敏度高、重复性好，可用于丹参提取物质量控制。Objective To develop an HPLC method for determination of 3 water - soluble components - danshensu, protocatechuic aldehyde and salvianolic acid B in the extract from Salvia miltiorrhiza Bge. Methods The analysis was carried on with a column of Hypersil ODS C lS （250 mm× 4.6 mm ,5μ m） by HPLC at 25℃, with gradient elution of 1% （v/v） acetic acid （A） - methanol （B） as the mobile phase. The flow rate was 1.0 ml · min^-1 and the detection wavelength was 281 nm. Results The linear ranges of danshensu, protocatechuie aldehyde and salvianolic acid B were 0.26 - 2.08 μg, 0.13 - 1.04 μg and 0.45 - 3.60 p.g respectively, and all the compounds showed good linearity（ r≥0. 999 6） in corresponding detected concentration range. The average recoveries were 97.76%, 99.56% , 99.68 % , and RSD was less than 3.4%. Conclusion The method is simple, sensitive, accurate, reproducible, and can be used to control the quality of extract from Salvia miltiorrhiza Bge..

关 键 词：丹参提取物 水溶性成分 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]