流动注射电化学发光分析法测定土霉素  

Determination of oxytetracycline hydrochloride in pharmaceutical samples by flow-injection electrochemiluminescence

作　　者：赵常志[1] 陶晟辰[1] 李莹[1] 李明华[1] 

机构地区：[1]生态化工教育部重点实验室青岛科技大学化学与分子工程学院,山东青岛266042

出　　处：《辽宁师范大学学报（自然科学版）》2008年第4期457-459,共3页Journal of Liaoning Normal University:Natural Science Edition

基　　金：精细化工国家重点实验室开放基金项目(KF0508)

摘　　要：基于土霉素在铂电极上增强三联吡啶合钌[Ru（bpy）3^2＋]的电化学发光信号,并结合流动注射分析技术,建立了1种测定土霉素的电化学发光分析新方法.在含有5.0×10^-5mol/L Ru（bpy）3^2＋的pH=8.0磷酸盐缓冲溶液中,采用1.15 V的应用电位和2.0 mL/min的流速,测定土霉素的检出限为1.30 mg/L（S/N=3）,线性范围为2.50-25.0 mg/L,相对标准偏差小于1.50%（n=9）,加标回收率为97.4%-104%.该法简单、快速、灵敏,现已成功地用于药物制剂中土霉素含量的测定.A new flow-injection electrochemiluminescence （ECL） analytical method of oxytetracycline has been proposed in this paper. It is based on a fact that oxytetracycline reacts with tris（2,2′-bipyridine）ruthenium（Ⅱ） [Ru（bpy）3^2＋] to produce enganced electrochemiluminescence signals. In the phosphate buffer solution of pH=8.0, the best ECL response was obtained at applied potential of 1.15 V in the present of 5.0×10^-5 mol/L Ru（bpy）3^2＋. The linear range of the present method is from 2.50 to 25.0 mg/L with a correlation coefficient of 0. 999 1 and the detection limit of 1.30 mg/L （S/N=3）. The method has been successfully applied to the determination of oxytetracycline in tablets. The relative standard deviation （RSD） was less than 1.50% （n=9） and the recoveries were from 97.4% to 104% for the determination of practical samples.

关 键 词：电化学发光 土霉素 流动注射分析 三联吡啶合钌 

分 类 号：O657.3[理学—分析化学]