大鼠皮肤和肌肉挫伤后细胞间黏附分子-1 mRNA表达变化  

Changes of ICAM-1 mRNA Expression in Rats′ Skin and Muscle after Contusion

作　　者：孙俊红[1] 杜秋香[1] 王小伟[1] 王英元[1] 

机构地区：[1]山西医科大学法医学院,山西太原030001

出　　处：《法医学杂志》2008年第6期407-410,共4页Journal of Forensic Medicine

基　　金：山西省留学回国人员基金资助项目(200528);山西省青年科技研究基金项目(2007021047);山西省教育厅高校科技开发项目(200713007)

摘　　要：目的应用实时荧光定量PCR技术检测大鼠皮肤和肌肉挫伤后组织中细胞间黏附分子-1(intercel-lular adhesion molecular-1,ICAM-1)mRNA的表达,分析其与损伤时间的关系。方法制备大鼠挫伤模型,于伤后0.5、1、6、12、18、24和30h取材,一步法提取总RNA,检测其完整性和质量浓度,逆转录合成第一链cDNA,以rpl32为内参基因进行荧光定量检测,采用△△Ct法比较其与正常皮肤、肌肉组织中ICAM-1mRNA表达的倍数关系。结果皮肤挫伤后ICAM-1mRNA的表达0.5h达到峰值,24h下降至正常对照组的7倍,随后再次升高;在肌肉组织中于损伤后0.5h表达显著增强,6h达到峰值,18h降至最低后再次升高。结论大鼠皮肤、肌肉挫伤后ICAM-1mRNA表达随损伤时间呈规律性变化,可望用于早期损伤时间推断。Objective To investigate the expression of intercellular adhesion molecule-1 （ICAM-1） mRNA in contused skin and muscle of rats and the relationship between the ICAM-1 expression and the wound age. Methods The samples were taken at 0.5, 1, 6, 12, 18, 24 and 30h after contusion of rats, respec- tively. Total RNA was extracted both from the skin and muscle samples of each group and reverse transcription polymerase chain reaction （RT-PCR） was conducted to synthesize the 1st strand cDNA. The amount of ICAM-1 mRNA in each sample was quantified using rp132 intrinsic fluorescent assay and compared by the 2（-Deha Delta Ct） method with that from control samples. Results After contusion the expression of ICAM-1 mRNA in skin increased rapidly and peaked at 0.5 h, at 24 h degraded to the amount that was seven times as much as the control group, then rised again. The expression of ICAM-1 mRNA in muscle increased significantly within 0.Sh and peaked at 6h, reached the minimum at 18h, then increased again. Conclusion It is suggested that ICAM-1 mRNA analysis may be useful for estimation of early wound age because of its time-related expression after contusion in skin and muscle.

关 键 词：法医病理学 挫伤 细胞间黏附分子 实时荧光定量聚合酶链式反应 损伤时间推断 

分 类 号：R978.1[医药卫生—药品] R685[医药卫生—药学]