关节炎慢性吗啡耐受大鼠脊髓背角谷氨酸天冬氨酸转运体1型的表达  

Expression of GLAST in spinal cord dorsal horn of arthritis rats with chronic morphine tolerance

作　　者：陈怡[1] 于泳浩[1] 石钊[1] 王国林[1] 

机构地区：[1]天津医科大学总医院麻醉科,天津300052

出　　处：《天津医科大学学报》2008年第4期412-415,共4页Journal of Tianjin Medical University

基　　金：天津市自然科学基金资助项目(06YFJMJC08600);天津市卫生局科技基金资助项目(06KZ51)

摘　　要：目的:在佐剂性关节炎大鼠模型基础上建立关节炎大鼠慢性吗啡耐受模型,观察兴奋性氨基酸转运体1型(GLAST)在关节炎慢性吗啡耐受大鼠脊髓背角表达变化,探讨GLAST在吗啡耐受形成机制中的作用。方法:将36只健康雄性SD大鼠随机分为6组(n=6),行鞘内置管。其中4组制成佐剂性关节炎模型,分别经鞘内给予生理盐水(A组)、吗啡10μg.kg-1(B组)、吗啡20μg.kg-1(C组)、吗啡10μg.kg-1+纳洛酮10μg.kg-1(D组),另外两组非致炎大鼠分别经鞘内给予生理盐水(E组)、吗啡20μg.kg-1(F组)。各组给药均为1日2次,连续7 d。动态检测大鼠50%缩爪阈值,以免疫组化方法检测脊髓背角GLAST表达。结果:B、C两组关节炎大鼠在鞘内给予吗啡第7天形成较稳定的吗啡耐受,其脊髓背角GLAST表达下调。结论:脊髓GLAST可能参与炎性痛大鼠慢性吗啡耐受的形成机制。Objective： To construct the rat model of arthritis-chronic morphine tolerance based on the adjuvant-induced arthritis model, observe the change of expression of GLAST in spinal cord dorsal horn of rats with chronic arthritis-morphine tolerance, and analyze the effect of GLAST on the development of morphine tolerance. Methods：The 36 healthy male SD rats were randomized into 6 groups and were implanted intrathecal catheters. Amongst, the rats in four groups were made into model of adjuvantinduced arthritis and were administered intrathecally saline （group A）, morphine 10μg·k^-1（group B）, morphine 20μg·k^-1（group C）, morphine 10μg·k^-1＋ naloxone 10μg·k^-1（group D）, respectively. The rats in the other two groups without arthritis were administered intratheeally saline （group E）, morphine 20μg·k^-1 （group F）, respectively. The drugs were administered twice daily for 7 days. Mechanical withdrawl threshold（MWT） of rats was examined to evaluate its behavior. Immunohistochemistry was used to detect the expression of GLAST in spinal cord dorsal horn. Results： Morphine tolerance was formmed stably in the arthritis rats of group B and group C after administering morphine for 7 days. The expression of GLAST in spinal cord dorsal horn was downregulated. Conclusion： Spinal GLAST may contribute to the development of morphine tolerance in rats with inflammatory pain.

关 键 词：慢性吗啡耐受 谷氨酸天冬氨酸转运体I型 佐剂性关节炎 大鼠 

分 类 号：R614[医药卫生—麻醉学]