炎性痛-吗啡耐受大鼠背根神经节辣椒素受体表达  

Expression of vanilloid receptor 1 in dorsal root ganglion in rats with inflammatory pain-morphine tolerance

作　　者：郑宇欣[1] 于泳浩[1] 王国林[1] 

机构地区：[1]天津医科大学总医院麻醉科,天津300052

出　　处：《天津医科大学学报》2008年第4期416-418,共3页Journal of Tianjin Medical University

基　　金：高等学校博士学科点专项科研基金资助项目(20060062002);天津市自然科学基金资助项目(06YFJMJC08600);天津医科大学科研基金资助项目(2007KY29)

摘　　要：目的:观察辣椒素受体(VR1)在慢性炎性痛-吗啡耐受大鼠背根神经节(DRG)表达变化,探讨VR1在吗啡耐受形成过程中的作用和机制。方法:将24只健康雄性SD大鼠制成关节炎模型,随机分为3组,鞘内给予吗啡10μg/kg组(A组),鞘内给予吗啡20μg/kg组(B组),鞘内给予生理盐水组(C组)。每日2次,连续7 d。以触觉测试包测试50%缩爪阈值,以免疫组化方法检测背根神经节VR1表达。结果:A、B两组在给药第5日形成较稳定的吗啡耐受,其背根神经节VR1表达阳性细胞数较生理盐水组多。结论:背根神经节辣椒素受体可能参与慢性炎性痛大鼠吗啡耐受形成过程。Objective： To investigate the change of expression of vanilloid receptor 1 in dorsal root ganglion in rats with chronic inflammatory pain-morphine tolerance and analysis the effect and the mechanism of vanilloid receptor 1 in morphine tolerance. Methods： The 24 SD rats with arthritis were randomly devided into three groups.Group A：given morphine 10μg/kg intrathecally two times a day. Group B：given morphine 20μg/kg intrathecally two times a day. Group C：given normal saline 10μl intrathecally two times a day.Mechanical withdrawl threshold（MWT） of rats was used to evaluate its behavior. Immunohistochemistry was used to detect the expression of vanilloid receptor 1. Results： Morphine tolerance was formmed in Group A and Group B after administering drugs for 5 days.The increased expression of vanilloid receptor 1 in dorsal root ganglion was observed in Group A and Group B compared with it in Group C. Conclusion： Vanilloid receptor 1 in dorsal root ganglion may participate in the formation of morphine tolerance.

关 键 词：吗啡耐受 辣椒素受体 关节炎 大鼠 

分 类 号：R614[医药卫生—麻醉学]