心肌线粒体腺苷酸配体门控钙离子释放通道  

A pathway for adenine nucleotide ligand gating Ca^(2+) efflux of myocadial mitochondria

作　　者：康少平[1] 李旭光[1] 董嘉良[1] 张艳君[1] 康英姿[1] 于公元[1] 

机构地区：[1]天津医科大学生物化学教研室,天津300070

出　　处：《天津医科大学学报》2008年第4期423-425,共3页Journal of Tianjin Medical University

基　　金：天津市教育委员会科研项目(200011)

摘　　要：目的:探讨心肌线粒体腺苷酸配体门控钙离子释放通道。方法:32只Wistar大鼠随机分成4组:ATP组、ADP组、NaCl组和咖啡因(Caffeine)组,每组8只。利用差速离心的方法提取心肌细胞线粒体。分别用ATP、ADP、NaCl和Caffeine启动线粒体Ca2+释放反应。紫外分光光度计监测线粒体的Ca2+释放。结果:ATP、ADP组线粒体Ca2+释放速度及最大释放量显著高于NaCl、Caffeine组(P<0.01),差异有统计学意义。结论:ATP、ADP能够触发心肌线粒体Ca2+释放,而NaCl、Caffeine则不可以。该实验数据提示心肌线粒体存在一种腺苷酸配体门控钙离子释放通道。Objective： To explore a pathway for adenine nucleotide ligand gating Ca^2＋ efflux of myocadial mitochondria. Methods： Thirty-two Wistar rats were randomly divided into four groups： ATP group, ADP group , NaCl group and Caffeine group （n=8）. Myocardial mitochondria were prepared with differential centrifugation method. ATP, ADP, NaCl and Caffeine were used to start the mitochondria Ca^2＋ releasing reaction respectively. The Ca^2＋ releasing was observed by UV spectrophotomete. Results： The rate and the maximum of myocardial mitochondria Ca^2＋ efflux of ATP and ADP groups were significantly higer than that of NaCl and Caffeine groups （P〈0.01）. Conclusion： ATP and ADP can trigger the Ca^2＋ efflux of myocardial mitochondria, but NaCl and Caffeine can not. The data suggests there is a pathway for adenine nucleotide ligand gating Ca^2＋ efflux in myocardial mitochondria.

关 键 词：心肌线粒体 钙离子 腺苷酸 配体门控 

分 类 号：Q7[生物学—分子生物学]