离子凝胶法制备壳聚糖载药微囊  

Preparation of drug-loaded chitosan microcapsules by ionotropic gelation

作　　者：高娴[1] 马世坤[2] 陈斌[1] 

机构地区：[1]天津医科大学生物化学教研室,天津300070 [2]天津医科大学化学教研室,天津300070

出　　处：《天津医科大学学报》2008年第4期463-465,共3页Journal of Tianjin Medical University

摘　　要：目的:采用离子凝胶法,建立制备壳聚糖载药微囊的方法,探讨最佳制备条件,考察药物电荷情况对微囊包封率的影响。方法:以三聚磷酸钠(TPP)为交联剂、牛血清白蛋白(BSA)为模型药物,以微囊包封率为优化指标,通过正交实验探索微囊最佳制备工艺;再分别以BSA(带负电)和异烟肼(带正电)为模型药物按照最佳工艺制备微囊,并比较两种微囊包封率。结果:最佳制备条件为:壳聚糖/TPP(w/w)3.75:1、pH值5.0、TPP滴加速度20滴/min、搅拌速度200 r/mim。带有负电荷的药物(BSA)微囊包封率明显高于带有正电荷的药物(异烟肼)微囊(P<0.001)。结论:离子凝胶法制备微囊方法简单,条件温和,比较适合用于带有负电荷药物微囊的制备。Objective： To prepare the chitosan microcapsules by the method of ionotropic gelation, explore the best preparation condition, detect the electric charge of the drug influence on the encapsulation efficiency. Methods： Used TPP as cross linking agent, BSA as model drug, encapsulation efficiency as evaluating indicator to explore the best preparation condition by orthogonal experiment, BSA （negative charge） and armazide （positive charge）microcapsules were prepared under the best condition, and the encapsulation efficiency of the two microcapsules were compared. Results： The best preparation condition was： chitosan/TPP （w/w）3.75：1, pH5.0, dropwise speed of TPP 20 drops/min, stir speed 200 r/min. The encapsulation efficiency of negative-charge-drug （BSA） microeapsules were obviously higher than positive-charge-drug （armazide） microcapsules （P〈0.001）. Conclusion： The ionotropic gelation method is simple, the condition is very mild, it is suitable for the preparation of negative-charge-drug loaded microcapsules.

关 键 词：壳聚糖 离子凝胶法 异烟肼 牛血清白蛋白 包封率 

分 类 号：R91[医药卫生—药学] Q78[生物学—分子生物学]