TCR分析技术作为辐射生物剂量计的可行性研究  

Feasibility Study on TCR as Rapid Radiation Biodosimeter

作　　者：段志凯[1] 郭万龙[1] 刘建功[1] 张淑贤[1] 

机构地区：[1]中国辐射防护研究院,太原030006

出　　处：《辐射防护通讯》2008年第5期32-35,共4页Radiation Protection Bulletin

摘　　要：以T淋巴细胞受体（TCR）为研究对象，探讨其作为辐射损伤生物剂量计的可行性。采集2名正常人外周静脉血，用不同剂量^60Coγ射线照射，分离淋巴细胞，用流式细胞仪检测TCR突变频率，建立剂量效应回归方程。结果表明，受到^60Coγ射线照射后，T淋巴细胞表面CD3、CD4表达情况发生改变，TCR突变频率在0—3Gy剂量效应关系良好。TCR is used as a goal of study to find the relationship between dose and effect. Peripheral blood, after being sampled from two normal persons, were exposed to ^60Coγ my at different doses, and then T cells were extracted and analyzed using flowcytometry. Result shows that exchange took place in expression of CD3 and CD, and that there is a relativly good relationship between dose and effect the range of 0 - 3 Gy.

关 键 词：TCR 生物剂量计 辐射损伤 

分 类 号：R144[医药卫生—公共卫生与预防医学]