^(99)Tc^m间接标记NGR-IFN-α2a的方法研究  

Study of Indirect ^(99)Tc^m Labelling NGR-IFN-α2a

作　　者：任炳秀[1] 汪静[1] 张路[2] 王喆[1] 杨卫东[1] 张英起[2] 李国权[1] 贺丽清[2] 

机构地区：[1]第四军医大学西京医院核医学科,陕西西安710032 [2]第四军医大学生物技术中心,陕西西安710032

出　　处：《同位素》2008年第4期226-230,共5页Journal of Isotopes

基　　金：国家自然科学基金资助项目(30570526)

摘　　要：以双半胱氨酸(L,L-ethylenedicystein,EC)作为双功能螯合剂,MDP作为转移螯合剂,采用两步法对NGR-IFN-α2a进行^(99)Tc^m标记,制备了^(99)Tc^m-EC-NGR-IFN-α2a,并对标记和非标记的NGR-IFN-α2a进行活性鉴定。结果显示,EC-NGR-IFN-α2a合成产额为87.5%,^(99)Tc^m-EC-NGR-IFN-α2a标记率86%,放化纯度96%,在2 h内,标记物体外较稳定;EC-NGR-IFN-α2a与^(99)Tc^m-EC-NGR-IFN-α2a的活性与NGR-IFN-α2a无差异。NGR-IFN-α2a was labelled with 99Tcm by two-step method used ethylenedicystein as a bifunctional chelating agent and MDP as a medium chelating agent. The bioactivities of EC-NGR-IFN-α2a and 99 Tcm_ EC-NGR-IFN-α2a were identified. It showed that the produc- tivity of EC NGR-IFN-α2a was 87.5%, the labelling yield rate and the radiochemical purity of 99Tcm- EC-NGR-IFN-α2a was 86% and 96%, respectively. In vitro, the radiolabelled compound had good stability,no bioactivity difference was found among EC-NGR-IFN-α2a, 99Tcm-NGR-EC-IFN-α2a and NGR-IFN-α2a.

关 键 词：99Tcm 间接标记 NGR 干扰素 

分 类 号：R817[医药卫生—影像医学与核医学] R978.7[医药卫生—放射医学]