人血浆萘哌地尔浓度的高效液相色谱法测定  

DETERMINATION OF NAFTOPIDIL IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

作　　者：李峰[1] 卢家红[1] 盛美萍[1] 江文德[1] 

机构地区：[1]上海医科大学临床药理研究所

出　　处：《上海医科大学学报》1998年第1期63-65,共3页Journal of Fudan University(Medical Science)

摘　　要：目的研究人血浆萘哌地尔的高效液相色谱（ＨＰＬＣ）测定。方法血浆样品依次用６ｍｏｌ／ＬＨＣｌ、ＮａＯＨ处理，乙醚提取２次。色谱分析柱为粒径５μｍ填料为ＥｃｏｓｐｈｅｒｅＣ１８，内标为美托洛尔，流动相为甲醇－乙腈－水－０．０２ｍｏｌ／ＬＫＨ２ＰＯ４（５５∶２０∶２２∶３），ｐＨ４．１０。荧光检测器激发波长２９０ｎｍ，发射波长３４０ｎｍ。结果最低检测浓度为１．０ｎｇ／ｍｌ，天内及天间的变异系数为１．１５％至４．７１％。结论此法价廉、灵敏、可靠性及重复性好，故适合国内条件下研究人体药代动力学。URPOSE To develop a high-performacne liquid chromatography (HPLC) method to determine the concentration of naftopidil in human plasm.METHODS Plasma samples were well putrified then extracted with ether two times. The internal standar was metoprolol. The analytical colum was a stainless steel column filled with 5 mircon C18 packing. The moblie phase was a maxture of methanol-acetonitrile-water-0.02mol/L KH2PO4 (55∶22∶20∶3, pH=4.10). Detection was performed at excitation wavelenth 290nm, emission wavelenth 340nm by a fluoresent detector.RESULTS The lowest detective limit was 1ng/ml. The precision and accuracy within-day and day-to-day ranged from 1.15% to 4.71%.CONCLUSIONS The results show the method developed by us is economic, sensetive, reliable and good enough to suit for the further pharmacokinetic study of naftopidil.

关 键 词：萘哌地尔 血药浓度 高效液相色谱法 

分 类 号：R972.4[医药卫生—药品] R969.1[医药卫生—药学]