HPLC-ELSD法测定人参茎叶中人参皂苷Rg_1、Re、Rd的含量  

Content determination of ginsenoside Rg_1,Re and Rd in stems and leaves of Panax ginseng by HPLC-ELSD assay

作　　者：胡杰[1] 秦雪梅[1] 郭小青[1] 张丽增[1] 

机构地区：[1]山西大学中医药现代研究中心,太原030006

出　　处：《山西医科大学学报》2008年第12期1095-1098,共4页Journal of Shanxi Medical University

摘　　要：目的建立HPLC-ELSD法测定人参茎叶中人参皂苷Rg1、Re、Rd含量的方法。方法采用Diamonsil(钻石)C18柱(200mm×4.6mm,5μm);流动相:乙腈(A)和水(B)按不同体积比混合,梯度洗脱程序:0-30min,21%A;30-35min,21%-33%A;35-60min,33%A;60-65min,33%-21%A;65-70min,21%A;流速为1.0ml/min,雾化温度35℃,蒸发温度50℃,载气N2,压力1.5Bar。结果人参皂苷Rg1、Re、Rd分别在1.06-7.95μg、1.94-14.55μg、1.26-9.45μg范围内呈良好的线性关系。3种人参皂苷的平均回收率分别为96.75%(RSD=1.99%)、97.77%(RSD=1.33%)、96.30%(RSD=1.26)。结论本方法灵敏、简便、准确,可用于人参茎叶中人参皂苷的含量测定。Objective To establish a HPLC-ELSD method for determining the contents of ginsenoside Rg1, Re and Rd in Panax ginseng. Methods With a Diamonsi/C18 column（200 mm × 4.6 mm, 5 μm）, acetonitrile（A）-water（B） was used as the mobile phase at a flow rate of 1.0 ml/min, and eluted in gradient mode（0 - 30 min, 21% A; 30 - 35 min, 21% - 33 % A; 35 - 60 min, 33 % A;60- 65 rain,33% -21%A;65- 70 min,21%A）. An evaporative light-scattering detector（ELSD） was used as the detector with atomization temperature of 35 ℃, evaporation temperature of 50 ℃, and N2 as carrier gas at the pressure of 1.5 Bar. Results The ginsenoside Rgl, Re and Rd had a good linearity in 1.06 - 7.95 μg, 1.94 - 14.55μg, 1.26 - 9.45 μg, respectively, and their average recoveries were 96.75 % （ RSD = 1.99 % ）, 97.77 % （ RSD = 1.33 % ）, 96.30 % （ RSD = 1.26 ）, respectively. Conclusion The method is sensitive, simple and accurate, and can be used for the content determination of ginsenoside.

关 键 词：HPLC-ELSD法 人参茎叶 人参皂苷RG1 人参皂苷RE 人参皂苷RD 

分 类 号：R927.2[医药卫生—药学]