反相高效液相色谱法测定犬血浆中甲硝唑浓度  

Determination of metronidazole concentration in dog plasma by RP-HPLC

作　　者：任国莲[1] 白克华[2] 吴婧[2] 张淑秋[2] 

机构地区：[1]山西医科大学药学院药剂教研室,太原030001 [2]山西医科大学药学院临床药学教研室

出　　处：《山西医科大学学报》2008年第12期1109-1111,共3页Journal of Shanxi Medical University

基　　金：山西省资助回国留学人员科研基金资助项目(2005029);太原市科技明星专项基金资助项目(07020425);山西医科大学青年基金资助项目(2006)

摘　　要：目的建立犬血浆中甲硝唑浓度反相高效液相色谱(RP-HPLC)测定方法。方法采用反相高效液相色谱法C18柱,以0.05mol/L的KH2PO4(用1mol/L的磷酸调节pH3.5)∶乙腈∶甲醇(130∶35∶35,V/V/V)作为流动相,流速0.6ml/min,检测波长317nm,柱温25℃,内标为替硝唑。结果RP-HPLC测定甲硝唑血药浓度的线性范围是0.2-20.0μg/ml(r2=0.9986),定量下限为0.2μg/ml,准确度在85%-115%范围内,其日内、日间精密度≤15%。结论本法可用于甲硝唑犬体内药动学研究的需要,并成功地用于口服甲硝唑片剂后犬体内的药动学研究。Objective To establish a RP-HPLC methood for determining the plasma concentration of metronidazole in dog. Methods A RP-HPLC method was developed. A column of C18 was used, with 0.05 mol/L KH2PO4 ： acetonitrile： methnol（ 130：35：35, V/V/V） as a mobile phase. The flow rate was 0.6 ml/min. The detective wavelength was set at 317 nm,and the column temperature was 25 ℃. Tinidazole was used as internal standard（IS）. Results The calibration curve was linear over the concentration range of 0.2 - 20.0 μg/ml. Lower limit of quantification（LLOQ） was 0.2μg/ml, and the accuracy of metronidazole was 85 % - 115 %. The precision RSD was less than 15 %. Conclusion The RP-HPLC is validated to guarantee a reliable determination of metronidazole concentration in dog plasma. The method can be applied to investigate the pharmacokinetics profile of metronidazole in dogs after oral administration.

关 键 词：甲硝唑 反相高效液相色谱法 血药浓度 

分 类 号：R969.1[医药卫生—药理学]