CD147在亲环素A诱导Jurkat细胞增殖、活化和趋化过程中的作用  

The role of CD147 in the proliferation, activation and chemotaxis of Jurkat cell induced by cyclophilin A

作　　者：陈翔[1] 粟娟[1] 匡叶红[1] 常静[1] 鲁慧[1] 邱英[1] 

机构地区：[1]中南大学湘雅医院皮肤科,长沙410008

出　　处：《中华血液学杂志》2008年第12期793-796,共4页Chinese Journal of Hematology

基　　金：基金项目：国家自然科学基金（30571682）;教育部新世纪优秀人才支持计划（NCET-05-0685）;湖南省杰出青年基金（06JJ-1005）;湘雅医院“百万人才战略工程”基金

摘　　要：目的探讨CD147分子在亲环素A（CyPA）诱导的Jurkat细胞增殖、活化和趋化过程中的作用。方法采用RT．PCR法和Western blot法证实CD147小干扰RNA（siRNA）转染Jurkat细胞的有效性。不同浓度CyPA（0．01、0．10、1．00、10．00nmol／L）及PBS作用于CD147 siRNA转染前后的Jurkat细胞，采用MTT法检测CyPA作用24和48h后细胞增殖水平；流式细胞术检测细胞表面T细胞活化标志分子CD25的表达；以Transwell小室法和基质黏附实验分别检测细胞趋化能力和黏附能力。结果CD147 siRNA转染的Jurkat细胞CD147 mRNA和蛋白表达水平较空转细胞显著下降；CyPA以浓度依赖性方式促进Jurkat细胞增殖，CyPA浓度为10．00nmol／L时促增殖作用最强，阻断CD147表达使CyPA促细胞增殖作用下降；CyPA可促进Jurkat细胞的活化，阻断CD147表达致CyPA促细胞活化能力下降；CyPA对Jurkat细胞有趋化作用，趋化指数为2．32，阻断CD147表达后细胞趋化活性显著降低；CyPA对Jurkat细胞的黏附能力无明显影响。结论CD147分子在CyPA诱导的Jurkat细胞活化、增殖和趋化过程中发挥作用。Objective To investigate the role of CDI47 in the proliferation, activation and chemotaxis of Jurkat cell induced by cyclophilin A（CyPA）. Methods CD147 mRNA and protein level siRNA trans-fected Jurkat cells were identified by RT-PCR and Western blot respectively. Jurkat cell, Jurkat-vector cell and Jurkat-CDl47siRNA ceils were treated with different concentrations of CyPA （0.01 nmol/L, 0.1 nmol/ L, l nmol/L, 10 nmoL/L） or PBS for 24 h and 48 h. Proliferation level was detected by MTT assay. CD25 was measured by flow cytometry. Transwell chamber was used to detect the chemotaxis. The effect of CyPA on the adhesive potential of Jurkat cell was studied by ceil-matrix adhesion assay. Results CD147 mRNA and protein level siRNA transfected cells were decreased significantly than that of control ceils. CyPA stimulated the proliferation of Jurkat cell in a dose-dependent manner, its effect peaked at 10 nmol/L CyPA. Blockage of CD147 expression decreased the proliferation level of Jurkat cell induced by CyPA. CyPA increased the activation rate of Jurkat cell, and blockage CD147 expression decresed the activation rate of the cell induced by CypA. CyPA showed a chemotatic activity on Jurkat cell, the chemotaxis index being 2.32, and the chemotactic ability was decreased after inhibition of CD147 expression. CyPA had no effect on adhesion of Jurkat cell to extracellular matrix. Conclusion CD147 plays a role in the proliferation,activation and chemotaxis of Jurkat cell induced by CyPA.

关 键 词：JURKAT细胞 亲环素A 基因 CD147 细胞增殖 细胞黏附 

分 类 号：R593.22[医药卫生—内科学] R392.12[医药卫生—临床医学]