流式细胞术在水生生物DNA含量和倍性分析中的应用  

The application of Flow cytometry on the DNA content and ploidy analysis of hydrophily organisms

作　　者：耿波[1] 孙效文[1] 

机构地区：[1]中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070

出　　处：《水产学杂志》2008年第2期21-24,共4页Chinese Journal of Fisheries

基　　金：国家计划(973)项目(No.2004CB117405)

摘　　要：利用流式细胞仪对鲤鱼、银鲫、黄颡、牙鲆等淡水和海水鱼类进行了DNA含量测定或倍性分析。通过几百个样品的测定和分析,认为流式细胞仪在DNA相对含量测定和倍性分析上结果比较稳定一致,可以用于大量样品的测定分析。同时对待测样品的保存、处理及上样量等进行了一系列比较研究,找出最好的保存方法、最简便的样品处理方法和最少的上样量。流式细胞仪在水生生物的遗传育种、种群分化等方面有很重要的应用价值。We used flowcheck to determine the DNA content and analyze the ploidy of many freshwater and seawater fishes such as, Carassius auratus gibelio,Pelteobagrus fulvidraco, Paralichthys olivaceus and so on. With several hundred samples analysis, it can be think that the results of determination with FCM are stable and consistent and FCM can be used to work on a great deal of samples. On the experiments, we also to do a series of research on the sample＇s conservation, deal with and the quantity of add samples to find the best way of conservation and the easiest deal measure with samples and the lowest quantity. FCM has important roles on the genetic breeding, stock differentiation of hydrophily organisms.

关 键 词：流式细胞术 DNA含量测定 倍性分析 

分 类 号：Q343.2[生物学—遗传学]