加味补肝汤对糖尿病大鼠坐骨神经中c—jun表达的影响  

Influence of jiaweibugan decoction on c-jun expression of sciatic nerve in experimental diabetic rats

作　　者：黄娟[1] 陈泽奇[1] 熊丽丽[1] 李杰玉[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,湖南长沙410008

出　　处：《中国医师杂志》2008年第11期1443-1446,共4页Journal of Chinese Physician

基　　金：湖南省卫生厅中医药科研经费资助（6202-38）;湖南省重点学科建设经费资助[湘教通（2001）179]

摘　　要：目的观察加味补肝汤对糖尿病大鼠坐骨神经中c-jun mRNA表达的影响，探讨其对实验性糖尿病大鼠周围神经病变的防治作用。方法以链脲佐菌素诱发糖尿病模型。分别于治疗后4周、8周两个时间点处死大鼠，用逆转录-聚合酶链反应（RT-PCR）技术测定坐骨神经中c-jun mRNA的表达，用黄嘌呤氧化酶法测定血清SOD活力。结果治疗后4、8周模型组大鼠c-jun mRNA表达明显高于正常对照组（P〈0．05），加味补肝汤组c-jun mRNA表达明显低于同期模型组（P〈0．05）；治疗后4、8周模型组大鼠SOD活力明显低于正常对照组（P〈0．05），加味补肝汤组SOD活力明显高于同期模型组（P〈0．05）。结论加味补肝汤对实验性糖尿病大鼠周围神经病变有一定的防治作用。Objective To observe the effect of Jiaweibugan decoction on c-jun mRNA expression of sciatic nerve in experimental diabetic rat and explore the preventive and therapeutic effects of Jiaweibugan decoction on peripheral neuropathy in experimental diabetic rats. Methods The diabetic rat model, was established by streptozotocin （STZ）. The rats were killed on the 4th or 8th week from the beginning of treatment, and c-jun mRNA of sciatic nerve was detected by reverse-transcriptase polymerase chain reaction （RT-PCR）. Serum SOD was determined by xanthine oxidase method. Results The results of RT-PCR showed that c-jun mRNA expression of the model group on the 4th or 8th week was higher than that of the normal control group （ P 〈0. 05） , while those in model rats treated by Jiaweibugan decoction were markedly lower than those in non-treated model rats（ P 〈0. 05）. The results showed that SOD of the model group on the 4th or 8th week was lower than that of the normal control group （ P 〈 0. 05） , while those in model rats treated by Jiaweibugan decoction were markedly higher than those in non-treated model rats （ P 〈 0.05）. Conclusion Jiaweibugan decoction has a preventive and therapeutic effect on peripheral neuropathy in experimental diabetic rats.

关 键 词：糖尿病神经病变 中药疗法 补益肝肾 汤剂 坐骨神经 原癌基因蛋白质c-jun 

分 类 号：R285.5[医药卫生—中药学] R259.872[医药卫生—中医学]