探讨受激活调节正常T细胞表达和分泌因子在阴道白色假丝酵母菌病小鼠模型中作用  

The studying of rantes in the nurine infection of VVC

作　　者：张洪文[1] 徐艳[1] 郑录清[1] 陈蒲香[1] 王丽琼[1] 

机构地区：[1]中南大学湘雅附二医院妇产科,湖南长沙410011

出　　处：《中国医师杂志》2008年第11期1459-1462,共4页Journal of Chinese Physician

摘　　要：目的通过对小鼠阴道白色假丝酵母菌病感染过程中受激活调节正常T细胞表达和分泌因子（RANTESF）的变化，探讨其在阴道白色假丝酵母菌病局部免疫中的作用。方法将雌性63只8—10周龄小鼠分为3组，分别为对照组、一次感染组，二次感染组，通过小鼠阴道种植白色假丝酵母菌制做小鼠阴道白色假丝酵母菌感染模型，分别于2、7、14d取小鼠阴道灌洗液及血液测RANTESF因子的含量，并留取部分阴道灌洗液做真菌培养。结果第一次感染组及第二次感染组血液及阴道灌洗液中的RAN-TESF因子的含量在感染第7天达到高峰，感染第2天及第14天含量无显著变化，含量均高于对照组。第二次感染组同时期RAN—TESF因子含量较第一次感染组显著增多。第一次感染组及第二次感染组同时期阴道灌洗液中RANTESF因子含量较血液中显著增多。阴道灌洗液真菌培养显示第一次、第二次感染组cfu计数于第7天达到高峰，第14天与第2天无显著差异。第二次感染组同时期真菌培养cfu计数比第一次感染组显著增多。结论RANTESF因子在抗白色假丝酵母菌的感染过程中发挥了作用，主要是通过阴道局部黏膜免疫发挥作用。且在再次感染过程中发挥较大作用。Objective To study the kinetic expression level of chemokines （RANTESF） in the murine infection of vulvovaginal candidiasis （VVC）, and explore the function of chemokines in local immunity of VVC. Method Sixty-three female Kunming mice, at 8 - 10 weeks of age, were used in this study. All animals were divided into three groups. The content variation of RANTESF in blood and vaginal fluids and CFU of vaginal fluid in each separate group of mice were detected at days 21 7, and 14 after infection. The first group was control group. The second group was infected only one time and the third group was infected twice. The results were analyzed with SPSS 13.0 statistical software. Results The content variation of RANTESF and CFU in vaginal fluid reached highest at days 7 in both the first and the second groups, as well as in the blood. There were no notable changes at days 2 and 14. The content variation in vaginal fluid or blood of the second group was higher than that in the first group after infection. Conclusion CMI, as a host defense mechanism, plays an important role in protecting against vulvovaginal candidiasis, especially in secondary infection. Local innate immunity is more important than systemic innate immunity for protection against vulvovaginal candidiasis. Cytokine about RANTES can promote innate immunity modulation; especially the local innate immunity modulation can promote the chemotaxis of RANTES.

关 键 词：RANTES 念珠菌 白色 念珠菌病 

分 类 号：R711.3[医药卫生—妇产科学]