他克莫司对UVA照射后HaCaT细胞表达ICAM-1的影响  

Effect of Tacrolimus in The Expression of ICAM-1 on The HaCaT Cell after UVA Exposure

作　　者：付兰[1,2] 余江云[2] 邹勇莉[1] 李玉叶[1] 罗雯[1] 赵万润[1] 何黎[1] 

机构地区：[1]昆明医学院第一附属医院皮肤科,云南昆明650032 [2]昆明市第一人民医院,云南昆明650032

出　　处：《中国皮肤性病学杂志》2008年第12期709-711,共3页The Chinese Journal of Dermatovenereology

基　　金：云南省教育厅科研基金项目(编号:07Z11040)

摘　　要：目的研究他克莫司(TM)对长波紫外线(UVA)照射后HaCaT细胞表达细胞间黏附分子-1(ICAM-1)的影响,以探讨TM治疗光敏性皮肤病的作用机制。方法采用ELASI和免疫组化法检测在不同强度UVA照射后,HaCaT细胞表达ICAM-1的情况。结果与未经UVA照射组细胞比较,未加TM组经UVA照射24h后,HaCaT细胞表达ICAM-1水平明显升高(P<0.01),而加入TM组的ICAM-1表达明显受到抑制(P<0.01)。结论TM对UVA照射后HaCaT细胞表达ICAM-1有抑制作用,提示对UVA引起的炎症有抑制作用。Objective To investigate the effect of tacrolimus （TM）in the expression of ICAM-1 on the HaCaT cell after UVA exposure and discuss the mechanism of action on tacrolimusin photoallergic dermatitis. Methods The expression of ICAM-1 on the HaCaT cell were measured by ELASI and immunohistochemistry after UVA exposure with different intensity. Results The group in that the UVA exposure was practiced for 24h had significantly more ICAM-1 expression on HaCaT cell that in the group without UVA exposure. The use of TM showed to inhibit significantly this the espression of ICAM-1. Conclusion The use of TM had an inhibitory effect of the expression of ICAM-1 on the HaCaT cell after the UVA expresure.

关 键 词：他克莫司(TM) 长波紫外线 HACAT细胞 细胞间黏附分子-1 

分 类 号：R392.12[医药卫生—免疫学]