从葡萄籽中分离纯化原花青素的研究  

Study of Extraction and Purification of Proanthocyanidins from Grape Seeds

作　　者：杨迎花[1] 李玉峰[1] 寇丽[1] 

机构地区：[1]天津科技大学理学院,天津300457

出　　处：《天津科技大学学报》2008年第4期52-55,共4页Journal of Tianjin University of Science & Technology

基　　金：天津科技大学科学研究基金资助项目(20060226)

摘　　要：研究了从葡萄籽中分离纯化原花青素的最佳浸提条件,经过正交实验,得出最佳浸提条件:乙醇体积分数60%,提取温度50,℃,料液比(g∶mL)1∶7.AB-8树脂较适合精制原花青素粗提物,最佳的柱分离条件:上样液pH=4,上样液流速2.0,BV/h,体积分数为50%乙醇溶液洗脱,洗脱流速1.0,BV/h.经AB-8树脂吸附精制,原花青素的纯度可达91.2%.The most optimum condition of extraction proanthocyanidins from grape seeds has been studied. The results show the optimum condition of extraction： the volume fraction of alcohol is 60%, the temperature of extraction is 50℃, and the ratio of feedstock and solvent is 1 ： 7. AB-8 is the most suitable resin to purify proanthocyanidins. The most optimum condition of column separation is that the solution pH value is 4, the solution flow rate is 2.0 BV/h, the concentration of al- cohol desorption is 50%, and the eluent flow rate is 1.0 BV/h. The purity of proanthocyanidin can be up to 91.2% after adsorption and purification of AB-8.

关 键 词：葡萄籽 原花青素 浸提 吸附 

分 类 号：O647.3[理学—物理化学]