荧光光谱分析家蝇幼虫抗菌肽MDL-2的结构特性  

Fluorescence Analysis of the Antibacterial Peptide MDL-2 from Musua Domestica Larvae

作　　者：宫霞[1] 凌庆枝[2] 乐国伟[3] 

机构地区：[1]上海商学院食品学院,上海200235 [2]浙江医药高等专科学校,浙江宁波315100 [3]江南大学食品学院,江苏无锡214122

出　　处：《食品与生物技术学报》2008年第6期90-92,共3页Journal of Food Science and Biotechnology

基　　金：中央级科研院所基础性专项资金工作项目--重点项目(2001DEA-20022)

摘　　要：研究了家蝇幼虫抗菌肽MDL-2的荧光光谱和淬灭剂对内源性荧光的影响。家蝇幼虫抗菌肽MDL-2在280 nm波长的激发光时,荧光光谱为Tyr残基和Trp残基共同提供,KI不能淬灭抗菌肽MDL-2的Trp残基的荧光,而Acr能淬灭几乎所有的Trp残基的荧光(f=0.74),说明Trp残基不是位于抗菌肽分子的表面,而是位于分子的内部。Effects the antibacterial of quenching agents on the intrinsic florescence and fluorescence spectroscopy of peptide MDL-2 from Musua Domestica Larvae were studied. The result showed that fluorescence spectra of MDL-2 excited to exhibit characteristic absorbency of Trp residues and Tyr residues at 280 nm. The Trp residue fluorescence of MDL-2 wasfft quenched by KI, but almost was quenched by acrylamide, respectively. The fraction of Trp quenched was obtained by the modified Stern-Volmer equation as 0.74. It was concluded that the Trp residues of MDL-2 were located in the inner district with negative dielectric instead of the surface.

关 键 词：抗菌肽 色氨酸残基 荧光光谱 荧光淬灭 

分 类 号：Q966[生物学—昆虫学]