扇贝裙边糖胺聚糖对U937泡沫细胞形成过程中TNF-α、IL-6和IL-8表达的影响  

Effects on the expression of TNF-α,IL-6 and IL-8 during the U937 foam cells formation of glycosaminoglycan from scallop skirt

作　　者：孙福生[1] 鞠传霞[2] 刘赛[2] 

机构地区：[1]青岛市市立医院,青岛266011 [2]青岛大学医学院,青岛266021

出　　处：《中国药科大学学报》2008年第6期563-565,共3页Journal of China Pharmaceutical University

基　　金：国家自然科学基金资助项目(No.30171157);山东省中医药科技发展计划项目资助(No.2005-016)~~

摘　　要：目的:研究扇贝裙边糖胺聚糖(SS-GAG)对氧化低密度脂蛋白(ox-LDL)诱导的U937泡沫细胞形成过程中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)表达的影响,以探讨SS-GAG抗动脉粥样硬化作用的机制。方法:采用U937细胞与80mg/Lox-LDL孵育48h建立U937泡沫细胞模型。将培养的U937细胞分为5组:正常细胞对照组、模型组(ox-LDL)和低、中、高浓度的SS-GAG药物组。分别检测不同处理组细胞分泌的TNF-α、IL-6和IL-8的含量。结果:培养的U937泡沫细胞中TNF-α、IL-6和IL-8表达量明显高于正常细胞组,而药物组中TNF-α、IL-6和IL-8表达量则明显降低,以800μg/mL药物组降低最为明显(P<0.01)。结论:SS-GAG能降低泡沫细胞形成过程中炎症因子TNF-α、IL-6和IL-8的表达从而发挥抗动脉粥样硬化的作用。Aim： To study the effects of glycosaminoglycan from scallop skirt （SS-GAG） on the expression of TNF-α, IL-6 and IL-8 during oxidized low-density lipoprotein （ox-LDL） induced U937 foam cells formation and the mechanism of anti-atherosclerosis action of SS-GAG. Methods： U937 cells were incubated with 80 mg/L ox-LDL for 48 h to establish a macrophage-derived foam cell model. In addition, U937 cells were divided into 5 groups： （1）control group, （2）model （ox-LDL） group, （3）ox-LDL ＋ 200 μg/mL SS-GAG group, （4）ox-LDL ＋ 400 μg/mL SS-GAG group, （5）ox-LDL ＋ 800 μg/mL SS-GAG group. In U937 foam cells, the expression of TNF-α, IL- 6 and IL-8 in medium was determined by ELISA. Results： In the model group, the expression of TNF-α, IL-6 and IL-8 increased significantly, while the expression of TNF-α, IL-6 and IL-8 significantly decreased in SS-GAG （200,400,800μg/mL） groups. Conclusion： SS-GAG is capable of attenuating the expression of TNF-α, IL-6 and IL-8 in U937 foam cells, which is probably attribued to the mechanism of its antitherosclerosis.

关 键 词：扇贝裙边糖胺聚糖 U937泡沫细胞 肿瘤坏死因子-Α 白细胞介素-6 白细胞介素-8 动脉粥样硬化 

分 类 号：R965[医药卫生—药理学] R285[医药卫生—药学]