适于木瓜属植物AFLP分析用DNA提取方法研究  

DNA Extraction Method for AFLP Analysis in Chaenomeles

作　　者：陈红[1] 张雷[1] 吕晓贞 孙永山 郑林[1] 臧德奎[1] 

机构地区：[1]山东农业大学林学院,山东泰安271018 [2]杭州市园林绿化工程有限公司,浙江杭州310016 [3]东营市东营区城市管理局,山东东营257000

出　　处：《北方园艺》2008年第12期161-163,共3页Northern Horticulture

基　　金：山东省优秀中青年科学家奖励基金资助项目(2004BS06003);山东农业大学博士基金资助项目(23215)

摘　　要：利用改进的CTAB法提取了29个木瓜属品种的基因组DNA,并进行了AFLP分析。结果表明：该方法提取的DNA溶液无色透明,紫外分光光度计测得A260/A280值为1.8-2.0,0.8%琼脂糖胶电泳为清晰的一条带,RNA去除干净,无降解现象;AFLP分析条带清晰,多态性好。说明该方法完全适于AFLP分析。The Genomic DNA samples of 29 cultivars in the genus Chaenomeles were isolated by modified CTAB method and then AFLP analysis was conducted. The result indicated that the DNA extracted by this method appeared as a clear band when electrophoresed in 0.8 % agrarose gel, and the RNA was eliminated completely. Measured the DNA by smart spec TM 3 000, the value of A260/A280 was 1.80-2.0. AFLP analysis indicated the primer （EcoRI-AAG ＋ MseI-CAA） produced clear polymorphie patterns. Therefore this method could be used for extracting ideal DNA samples and be suitable for AFLP.

关 键 词：木瓜属 DNA提取 AFLP 

分 类 号：S685.99[农业科学—观赏园艺] Q946.2[农业科学—园艺学]