家蚕品种金秋及其杂交亲本的蛋白质表达谱分析  

Analysis of Protein Expression Patterns of Silkworm Jinqiu and Its Cross Parents

作　　者：余芳[1,2] 杨惠娟[1] 李建营[1] 丁农 周仲华[1] 叶键[1] 张金卫 段家龙[2] 钟伯雄[1] 

机构地区：[1]浙江大学动物科学学院,杭州310029 [2]安徽农业大学生命科学学院,合肥230036 [3]湖州市农业科学院,浙江湖州313000

出　　处：《中国农业科学》2008年第12期4194-4200,共7页Scientia Agricultura Sinica

基　　金：国家高技术研究发展计划“863”项目(2006AA10A118);国家重点基础研究发展计划“973”项目(2005CB121003);浙江省科技厅重点科研项目(2005C22046)

摘　　要：【目的】探讨家蚕杂交育成品种及其亲本蛋白水平的差异,从蛋白水平分析育成品种的基因结构,为阐明杂交育种成败的分子机理创造条件。【方法】采用蛋白质组研究技术,分析杂交亲本及其育成品种的丝腺、血淋巴和中肠的蛋白质表达谱。【结果】3种组织器官中育成品种金秋与两个亲本的匹配蛋白斑点比例只达到70%左右,剩余30%左右是各个品种的特异蛋白斑点;在匹配蛋白斑点中,还有9%～24%的蛋白表现上调和下调。这些特异蛋白可能是两个亲本的基因在杂交育成品种中产生了互作而形成的新蛋白,也可能是对相同的蛋白进行了不同的修饰而获得的修饰蛋白。【结论】新品种的育成,除了汇集亲本的优良基因外,还有赖于基因互作产生的新功能蛋白的作用。【Objective】To explore the protein expression differences between the improved cross breeding race Jinqiu and its parents,the protein expression patterns were analyzed among them to discuss the gene construction and make a base for illuminating the mechanisms of successful cross breeding in silkworms.【Method】Protein samples from hemolymph,silk gland and midgut were isolated by technology of proteome research.【Result】The matched proteins between Jinqiu and its cross parents were both about 70% in the three kinds of tissue.The remaining 30% proteins were special to them,respectively.In the matched proteins there were also 9%-24% proteins were up-regulated or down-regulated expression.These special proteins may be the new proteins which come from the genic interaction of cross parents’ genes in cross breeding or modifying proteins which produced from the different modifications of the same original proteins.【Conclusion】The results indicate that it is important for a successful of cross breeding a new breed relying on the actions of some new produced functional proteins from genic interaction in addition to marshaling excellent genes of cross parents.

关 键 词：家蚕 蛋白质组 杂交育种 丝腺 血淋巴 中肠 

分 类 号：S881[农业科学—特种经济动物饲养]