猪源支气管败血波氏杆菌的分离鉴定及生物学特性研究  

Isolation, Identification and Characterization for Bordetella bronchiseptica from Diseased Pigs

作　　者：赵战勤[1,2] 裴洁[1] 薛云[1,2] 汤细彪[1] 吴斌[1] 何华[1] 周学利[1] 程相朝[2] 何启盖[1] 陈焕春[1] 

机构地区：[1]华中农业大学动物医学院,农业微生物学国家重点实验室,武汉430070 [2]河南科技大学动物科技学院,河南洛阳471003

出　　处：《中国农业科学》2008年第12期4209-4217,共9页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目(30471292);国家科技支撑计划(2006BAD06A12,2006BAD06A18)

摘　　要：【目的】探讨2003～2006年间支气管败血波氏杆菌(Bordetella bronchiseptica,Bb)在中国湖北等十余省市猪群中的流行病学分布和协同感染特性并对部分菌株进行生物学特性研究。【方法】采用病原菌的形态学观察、生化鉴定和PCR鉴定,从2 057份有肺炎或萎缩性鼻炎症状猪的肺脏等病料组织中分离出190株Bb和不同数量的其它共感染菌。经生化鉴定,药敏试验、血凝试验及动物试验等方法进行检测和分析。【结果】在中国湖北等十余省市的猪群中,不同省份Bb的总分离率介于7.3%～14.1%之间;不同年份Bb的总分离率介于7.3%～11.8%之间;2003～2006年间的Bb总分离率为9.2%;Bb的分离率与猪日龄有一定的关系。Bb最常见的共感染菌依次是链球菌(55.9%)、副猪嗜血杆菌(50.0%)、大肠杆菌(43.1%)、巴氏杆菌(25.5%)、绿脓杆菌(17.6%)和沙门氏菌(11.8%)。在43份有典型萎缩性鼻炎症状猪的病料中,分离出22株Bb、7株产毒素巴氏杆菌和6株绿脓杆菌;在分离出产毒素巴氏杆菌的7份病料中均同时分离出了Bb,而其中6份又同时分离出了绿脓杆菌。对2003年分离的31株Bb进行药敏试验、红细胞凝集试验和乳鼠皮肤坏死试验。结果表明:各菌株对15种药物的耐药谱不同,但对卡那霉素、庆大霉素、多粘菌素B、环丙沙星和舒普深高度敏感;各菌株均可凝集猪和羊的红细胞且能不同程度导致乳鼠皮肤坏死。【结论】在中国湖北等十余省市的猪群中,Bb与其它病原菌的共感染情况非常严重,且在萎缩性鼻炎的病猪中检出率最高。【Objective】The epidemiological distribution,synergistic infection,antibiotic sensitivity and other biological characterization of Bordetella bronchiseptica（Bb） were determined in this paper.【Method】Up to 190 Bb strains were isolated from 2 057 lesion lungs of pigs with clinical signs of pneumonia or atrophic rhinitis while different concurrent infection bacteria species were also separated from the same samples.【Result】These microorganisms were further confirmed by corresponding specific bio-chemistry assay and polymerase chain reaction（PCR）.The isolation ratios in the samples that were collected from different provinces and at the different time points（year） ranged from 7.3% to 14.1% and 7.3% to 11.8% respectively.The average isolation ratio between the years of 2003 to 2006 was 9.2%.The isolation ratios of identified co-infection bacterial species were 55.9%（57 strains）,50.0%（51 strains）,43.1%（44 strains）,25.5%（26 strains）,17.6%（18 strains）and 11.8%（12 strains） for Streptococcus,Haemophillus parasuis,E.coli,Pasterella multocida,Pseudomonas aeruginosa and Salmonella,respectively.Twenty-two Bb strains were isolated from 43 samples of lesion lungs and nasal swabs of pigs with typical clinical signs of atrophic rhinitis while 7 toxigenic Pasteurella multocida（T＋Pm） strains and 6 Pseudomonas aeruginosa（Pa） strains were also separated from the same samples.Among these,7 T＋Pm strains were isolated from the 7 of 43 samples,from which 7 Bb strains and 6 Pa strains were separated,simultaneously.Thirty-one Bb stains isolated in 2003 were selected from total 190 strains for determination of drug resistance,erythrocyte agglutination and induction of skin necrosis in suckling mice.The results showed that although the diverse resistance spectra to 15 antibiotics were observed,the tested strains were highly sensitive to kanamycin,gentamycin,polymyxin B,ciprofloxacin,sulperazone,agglutinated swine and sheep erythrocytes and,to different extent,produced skin necrosis in

关 键 词：支气管败血波氏杆菌 流行病学 协同感染 毒力因子 

分 类 号：S852.61[农业科学—基础兽医学]