大豆胚尖再生体系的研究  

Study on the regeneration system of soybean embryonic tips

作　　者：张东旭[1] 张洁[1] 商蕾[1] 张蕴伟[1] 杨春燕 王冬梅[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001 [2]河北农业科学院粮油作物研究所,河北石家庄050031

出　　处：《河北农业大学学报》2008年第4期7-13,共7页Journal of Hebei Agricultural University

基　　金：河北省科技攻关计划项目(No．042401116D-1)

摘　　要：为建立良好的大豆遗传转化受体系统,本研究以河北省8个优质大豆品种为材料,探讨了激素种类、浓度、诱导时间等因素对胚尖生长过程的影响,并对胚尖再生体系与传统的子叶节再生体系进行了比较。结果表明:经0.5～1.0 mg/L 6-BA诱导5d大豆的胚尖出芽率、植株再生率及胚尖丛生芽数等综合指标较好,植株再生率最高达84.1%。胚尖再生体系与子叶节再生体系的比较表明:以胚尖为外植体的大豆再生体系具有再生率高、操作简便、周期短、重复性好的优点。This experiment established an effective regeneration system with high- quailty soybean of Hebei by exploring the effects of the types, inducing time and the concentration of hormones on explants of embryonic tips, and comparing the differences between two regeneration systems of soybean from cotyledonay nodes and embryonic tips. The results indicated that the optimal concentration of 6 - BA from 0.5 to 1.0 mg/L,and the optimal inducing time was 5 d. The regeneration rate of embryonic tips was obviously higher than cotyledonary nodes. The regeneration system of soybean embryonic tips had the merit of higher regeneration rate, simpler and more convenient operation, shorter cycle and better repeatability.

关 键 词：大豆 胚尖 子叶节 再生体系 

分 类 号：S336[农业科学—作物遗传育种]