兔抗小麦钙调素抗血清的制备及其纯化  

Preparation and purification of rabbit anti-wheat calmodulin antiserum

作　　者：黄晨[1] 侯春燕[1] 王冬梅[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001

出　　处：《河北农业大学学报》2008年第6期55-58,共4页Journal of Hebei Agricultural University

基　　金：国家自然科学基金资助项目(No.30671244);植物生理学与生物化学国家重点实验室开放课题资助项目(No.PPB04006);河北省自然科学基金资助项目(No.303180No.C2005000220No.C2007000515)

摘　　要：为制备兔抗小麦钙调素抗血清并对其进行纯化,首先从小麦叶片中提取钙调素蛋白,对其初步纯化后免疫家兔,制备得到兔抗小麦钙调素抗血清,再以大肠杆菌中诱导表达的拟南芥钙调素蛋白作为抗原,利用PVDF膜结合法纯化得到了兔抗小麦钙调素抗血清,并通过Western Blotting对所纯化的多克隆抗体进行了鉴定。To prepare and purify rabbit anti-wheat calmodulin antiserums, calmodulin protein was extracted from wheat leaves for immuned rabbits after preliminary purification. Then Arabidopsis thaliana calmodulin protein was used by E. coli induced expression as antigen to purify rabbit anti- wheat calmodulin antiserum by PVDF membrane binding. The purified polyclonal antibody was identified by the Western Blotting.

关 键 词：钙调素 多克隆抗体 纯化 WESTERN BLOTTING 

分 类 号：Q942.6[生物学—植物学]