光诱导c-fos基因在母鸡中脑的表达  

The expression of c-fos gene induced by light stimulation in hen mesencephalon

作　　者：陈坤[1] 胡满[1] 狄慧[1] 程金金[1] 郭洁[1] 

机构地区：[1]河北农业大学动物科技学院,河北保定071001

出　　处：《河北农业大学学报》2008年第6期91-94,共4页Journal of Hebei Agricultural University

基　　金：河北省自然科学基金(C2007000537);河北农业大学校长基金(20040416);河北省留学回国人员科技活动项目择优资助基金(2006173)

摘　　要：为了解光照对母鸡生产性能的影响,采用c-fos法探讨光信息在母鸡中脑的传导通路,将鸡右眼遮光7 d后接受20 lx的光照刺激1.5 h,暗适应1.5 h后灌流固定,取脑制作石蜡切片,采用免疫组化方法检测c-fos基因在中脑的表达。结果显示:中脑内有大量Fos样免疫反应阳性神经元,主要见于左侧,分布于中脑SGC层、峡核(大细胞部Imc、小细胞部Ipc)、峡视核(ION)、中脑外侧核背侧部(MLd)、红核(RN)、滑车神经核(nIV)。表明光照信息主要通过视网膜经离顶盖通路传达至母鸡中脑,同时顶盖-峡核回路也起重要作用。The pathway of optical information in hen mesencephalon was investigated using c-fos method, to provide basic data for understanding the mechanism of the effect of light on egg - lay- ing. Unilateral eyes of hens were kept dark for seven days before they accepted light stimulation of 20 Ix for 1.5 hours and dark adaptation 1.5 hours. After perfusion with fixative, their brains were removed from skulls and made into continuous paraffin sections. Using immunohistochemical meth- od, expression of c-fos gene in mesencephalon was examined. The result showed that a number of Fos-like immunoreactive positive neurons were observed in mesencephalon, being mainly present in left stratum griseum centrale （SGC） of tectum, isthmi pars principals （Imc, Ipc）, nucleus isthmo - opticus （ION）, nucleus mesencephalicus lateralis （pars dorsalis, MLd）, nucleus raphes （RN）, and nucleus nervi trochlearis （nIV）. The results suggested that though the optical information is mainly conveyed to mesencephalon through the tectofugal pathway, the tectoisthmi circus also plays an important role in this course.

关 键 词：中脑 光刺激 C-FOS基因 免疫组织化学 母鸡 

分 类 号：S852.16[农业科学—基础兽医学]