PDT诱导的凋亡细胞对巨噬细胞NO合成的影响  

Effects of PDT-treated Apoptotic Cells on NO Synthesized in Macrophages

作　　者：宋盛[1] 邢达[1] 周非凡[1] 陈伟[1] 

机构地区：[1]华南师范大学激光生命科学研究所暨激光生命科学教育部重点实验室,广东广州510631

出　　处：《激光生物学报》2008年第6期711-715,共5页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(30470494;30627003);广东省自然科学基金项目(7117865)

摘　　要：NO作为细胞间信息传递的重要调节因子,在肿瘤的发生、发展以及转移过程中被广泛研究。一氧化氮合酶是合成NO的关键酶,诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)通常在应激、荷瘤等病理状态下被激活,产生大量NO。NO具有细胞毒性,与机体免疫反应及细胞凋亡有关,在许多致癌和抑癌机制中扮演着重要角色。实验探讨了光动力学疗法(photodynamic therapy,PDT)处理产生的小鼠乳腺癌凋亡细胞对巨噬细胞产生NO的影响,从而确定活化的巨噬细胞在肿瘤生长中的作用。Nitric oxide as an important regulatory factor in signal transfer between cells has been wildly studied for generation, development, and metastasis of tumors. Nitric oxide synthase is a key enzyme in the synthesis of nitric oxide. However, inducible nitric oxide synthase （iNOS） is usually activated under pathological conditions, such as stress and cancer, then produce high levels of nitric oxide, which contribute to tumor cytotoxicity. In addition, increased nitric oxide （NO） production by iNOS has been associated with the host immune response and cell apoptosis, which play an important role in many carcinogenesis and anti-carcinoma mechanisms. This study focuses on the NO production in macrophages, induced by mouse breast carcinoma apoptotic cells treated by PDT in vitro, and then determines the effect of activated macrophages in tumor cells growth.

关 键 词：光动力学疗法 凋亡细胞 巨噬细胞 NO 

分 类 号：Q274[生物学—细胞生物学]