血卟啉单甲醚在A549肺癌细胞内亚细胞分布的动态变化  

Dynamical Change of Intracellular Distribution of Hematoporphrin Monomethyl Ether in A549 Cells

作　　者：戴维德[1] 李晓松[2] 曾晶[2] 刘凡光[2] 顾瑛[2] 

机构地区：[1]卫生部北京医院超声科,北京100730 [2]解放军总医院激光医学科,北京100853

出　　处：《激光生物学报》2008年第6期725-728,共4页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(60078021)

摘　　要：目的:探讨血卟啉单甲醚(Hematoporphyrin monomethyl ether,HMME)在A549肺癌细胞内亚细胞分布的动态变化。方法:传代培养A549肺癌细胞,分别与光敏剂HMME孵育2 h和12 h。应用由荧光显微镜及电感耦合器材(Charge-coupled device,CCD)组成的高分辨率荧光显微成像系统,结合荧光探针标记技术,采用细胞器-细胞荧光强度比值法研究HMME在不同时间的亚细胞分布情况。结果:在2 h和12 h两个孵育时间点高尔基体的平均荧光强度比值(J1/J2)值都最高;随着孵育时间延长,A549细胞的四种细胞器J1/J2值都升高且溶酶体幅度最大。结论:孵育时间是影响HMME亚细胞分布的一个重要因素。随着孵育时间的延长,A549肺癌细胞各细胞器吸收HMME能力逐渐增强,尤以溶酶体显著。Objective： To discuss the dynamical change of intracellular distribution of Hematoporphrin monomethyl ether in A549 cells. Methods： A549 cells were subcuhured, then incubated with HMME for 2 h and 12 h. High-resolution Fluorescence Microimaging system consisting of fluorescence microscope and CCD and labeling technology of fluorescence probes were applied and Organelle-cell fluorescence intensity ratio analysis was adopted to study subcellular localization of HMME in various situation. Results： The average fluorescence intensity ratio （J1/J2 ） of Golgi is highest both at 2 h and 12 h. With incubating time prolonging, J1/J2 of A549 cells＇ four organelles were increasing, and the increasing of J1/J2 of A549 cells＇ lysosome was higher than that of other oranelles of A549 cells＇. Conclusions： Influence of incubating time on subcellular distribution of HMME is significant. With incubating time prolonging, four organelles＇ ability to absorb HMME is enhancing in A549 cells, especially does lysosome.

关 键 词：孵育时间 细胞种类 血卟啉单甲醚 细胞内分布 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]