环氧化酶-2对肿瘤细胞的作用机理研究  

Investigation into the Mechanism of Cyclooxygenase-2 Inhibitor on Tumor Cell

作　　者：宋佳兴[1] 邢达[1] 魏言春[1] 汪献旺[1] 

机构地区：[1]华南师范大学激光生命科学研究所暨激光生命科学教育部重点实验室,广东广州510631

出　　处：《激光生物学报》2008年第6期729-732,774,共5页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(30470494;30627003);广东省自然科学基金项目(7117864)

摘　　要：主要检测环氧化酶-2对肿瘤细胞的影响,分析讨论了环氧化酶-2引起细胞凋亡的潜在机制。不同浓度的环氧化酶-2处理肿瘤细胞的结果表明:环氧化酶-2对肿瘤细胞的抑制作用为时间和浓度依赖型。转染Bax-YFP与DsRed-Mit质粒并经环氧化酶-2处理3 h后的结果显示:肿瘤细胞中Bax明显聚集,细胞形态发生变化。实验证明环氧化酶-2促使肿瘤细胞凋亡是经过线粒体途径而发生的。This study mainly investigated the effect of cyclooxygenase-2 （ COX-2 ） inhibitors on tumor cells in vitro, and analyzed the potential mechanism that COX-2 inhibitors induced tumor cells apoptosis. After cell treated with different concentrations of COX-2 inhibitors, the results showed that COX-2 inhibitors could elicit the inhibition on tumor cells in an obviously time- and dose-dependent manner in vitro. Confocal imaging of the cells transfected with YFP-Bax and DsRed-Mit plasmids demonstrated that Bax aggregated on the mitochondria and the morphology of tumor cell began to change after COX-2 inhibitor treatment for 3 h. These results suggest that COX-2 inhibitors could induce cell apoptosis via the mitochondria pathway.

关 键 词：环氧化酶-2 塞来昔布 肿瘤 凋亡 BAX 

分 类 号：Q55[生物学—生物化学] Q279