用cDNA-AFLP及其改进的方法分析茶树花发育过程中的基因表达(英文)  

Transcriptional Profiling by cDNA-AFLP and Its Modified cDNA-AFLP Technique Reveals Gene Expression of Tea (Camellia sinensis) during Flower Bud Development Stage

作　　者：叶爱华[1] 余梅[1] 朱林[1] 江昌俊[1] 王朝霞[1] 韦朝领[1] 李叶云[1] 

机构地区：[1]安徽农业大学农业部茶叶生物化学与生物技术重点开放实验室,安徽合肥230062

出　　处：《激光生物学报》2008年第6期733-738,共6页Acta Laser Biology Sinica

基　　金：National Key Technology R & D Program(2008BAC0B03);National Natural Science Foundation of China(30871568);The key program of Scientific Research Fund of Anhui Provincial Education Department(2004kjl37zd)

摘　　要：利用cDNA-AFLP及其改进的cDNA-AFLP方法,分析茶树花发育过程中的基因表达。其发育过程中的基因表达可以分为3类:未成熟阶段发育特异基因;成熟阶段发育特异基因;茶树花发育过程中均表达的基因。利用改进的cDNA-AFLP方法,我们获得编码花药发育特异基因:pollen coat protein(Pcp)。用cDNA-AFLP方法,我们获得7个已知功能基因分别编码Cytchrome(P450),beta-primeverosidase,Dnaj-like protein,anthranilate phosphoribosyl transferase(AnPRT),Ribulose-1,5-bisphosphate carboxylase/oxygenasesmall subunit(RubpS),alpha-tubulin和Carbonic anhydrase。用RACE方法获得pollen coat protein(Pcp),DnaJ-like protein和Ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit等3个基因的全长,并已提交GenBank。A comprehensive transcriptome analysis by means of cDNA-amplified fragment length polymorphism （AFLP） and modified cDNA-AFLP technique were performed in order to gain further understanding of the development in molecular level of tea reproductive organs ： tea floral bud. Fragments detected among the young and the mature tea floral bud were grouped into three differential expression patterns ： （ Ⅰ ） The young-specific expressed fragments; （ Ⅱ ） Fragments detected only in the mature tea floral bud; （ Ⅲ ） Fragments occurring in both. Some transcript-derived fragments （TDFs） were isolated by cDNA-AFLP and its modified technique. The majority of the TDFs are still function unknown according to BLAST alignment search in public data bases. Some of them showed homology with known genes. They are anthranilate phosphoribosyl transferase （AnPRT） , beta-primeverosidase, Cytchrome （ P450 ）, DnaJ-like protein, Ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit （RbcS）, alpha-tubulin and Carbonic anhydrase by cDNA-AFLP technique. We isolated pollen coat protein gene（Pcp） by modified cDNA-AFLP method. The full length of three genes, pollen coat protein （Pcp）, DnaJ-like protein and RbcS, have been cloned and submitted to Gen Bank.

关 键 词：茶 CDNA-AFLP 花芽 

分 类 号：Q786[生物学—分子生物学]