功能红细胞研究的拉曼光谱技术与进展  

Technique and Advance of Raman Spectroscopy on Functional Erythrocyte Study

作　　者：陈秀丽[1,2] 王桂文[2] 刘军贤[1] 周金旺[1] 

机构地区：[1]广西师范大学物理与电子工程学院,广西桂林541004 [2]广西科学院生物物理实验室,广西南宁530003

出　　处：《激光生物学报》2008年第6期845-852,共8页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(30660063);广西科学院科技创新项目(桂科院研0702)

摘　　要：红细胞在人体新陈代谢和物质运输方面起着非常重要的作用。作为一种无损、快速而灵敏的光谱学检测方法,拉曼光谱技术在生物医学领域的应用日渐广泛。本文综述了近年来拉曼光谱技术在红细胞及血红蛋白研究中的应用进展,详尽评述当前研究功能红细胞中较有优势的拉曼技术—显微拉曼光谱技术和光镊拉曼光谱技术,并对其技术的改进做出总结,以期为利用激光拉曼光谱研究红细胞提供较系统的参考。Erythrocyte plays very important roles in metabolism and substance transportation for human body. Raman spectroscopy, which offers a noninvasive, nondestructive, fast, and water-insensitive technique, is widely applied in biology and medicine. With a goal of providing systemic reference on the erythrocyte study by laser-Raman spectroscopy, this paper reviews the application and development of Raman spectroscopic technique on the research of erythrocyte and hemoglobin in recent years. The leading technology, confocal micro-Raman spectroscopy and laser tweezers Raman spectroscopy, including technical improvement, in the functional erythrocyte study is focused on mainly.

关 键 词：拉曼光谱 功能红细胞 血红蛋白 共振机制 

分 类 号：O657.37[理学—分析化学]