脯氨酸和硝酸银对农杆菌转化香蕉的影响研究  

The Effects of Proline and Silver Nitrate on Agrobacterium-mediated Transformation in Banana(Musa spp.)

作　　者：赵巧阳[1] 赖钟雄[1] 

机构地区：[1]福建农林大学园艺植物生物工程研究所,福州350002

出　　处：《生物技术通报》2008年第6期97-100,105,共5页Biotechnology Bulletin

基　　金：国家科技部科技支撑计划;福建省重大科技专项(2004NZ02-2)

摘　　要：如何提高转化效率是植物遗传转化中的一个关键问题。已经发现有许多物质如乙酰丁香酮(AS)等许多酚类化合物、葡萄糖等能够促进农杆菌介导的遗传转化。试验在农杆菌转化香蕉茎尖薄片的过程中附加化合物脯氨酸、硝酸银,通过对GUS基因表达的分析发现,这2种化合物均能明显地提高香蕉茎尖薄片的转化率,其中浓度为2mg.L-1的脯氨酸及2mg.L-1的硝酸银效果最佳。How to improve transformation frequency is a key problem in plant transformation mediated by Agrobacterium tumefaciens,and substances such as phenols compounds（acetosyringone） and glucose can promote Agrobacterium-mediated transformationin in plants.In this experiment,the effects of two compounds of proline and silver nitrate on banana transformation of the thin cross-sections via Agrobacterium tumefaciens,were compared.The GUS gene expression indicated that the two compounds could improve transformation frequency obviously and the best concentration was 2mg·L^-1 for proline or 2mg·L^-1 for silver nitrate.

关 键 词：香蕉 农杆菌 遗传转化 脯氨酸 硝酸银 

分 类 号：S668.1[农业科学—果树学] Q943.2[农业科学—园艺学]