利用差异显示法研究刺参表皮再生相关基因  

Studies on Genes of Cuticle Regeneration Using Differential Display Method in Apostichopus japonicus

作　　者：陈秋实[1] 李霞[1] 段晶晶[1] 周伯文[1] 

机构地区：[1]大连水产学院农业部海水增养殖学与生物技术重点开放实验室,大连116023

出　　处：《生物技术通报》2008年第6期124-127,131,共5页Biotechnology Bulletin

基　　金：国家自然科学基金资助项目(30371099);辽宁省自然科学基金资助项目(20052139)

摘　　要：应用mRNA差异显示法对刺参(Apostichopus japonicus)正常表皮组织和手术后再生的表皮组织的基因的差异表达进行了分析。利用经本实验室摸索改进的mRNA差异显示体系,获得了若干差异片段,将其测序结果在GenBank数据库中比对发现,其中一个差异片段与CD151基因具有较高的同源性,并且采用RT-PCR的方法对差异片段进行了验证。进一步分析表明,该基因可能与表皮的再生密切相关。Differential display reverse transcript polymerase chain reaction（DDRT-PCR）was applied to study gene expression in normal cuticle and regeneration cuticle of the sea cucumbers（Apostichopus japonicus）.mRNA differential display system was improved and several differential expression cDNAs were obtained using this method.Blast results from GeneBank showed that one of these fragments was highly homologous with partial sequence of CD151 gene,and it was also proved by RT-PCR.Sequence analysis showed that the gene may relate to regeneration.

关 键 词：刺参 表皮再生 MRNA差异显示 

分 类 号：Q3[生物学—遗传学]