AFLP分子标记技术的改进——内切酶EcoRⅠ/TruⅠ组合与EcoRⅠ/MseⅠ组合的比较  

Improvement of AFLP Molecular Marker Technique—Comparion between Restriction Enzyme Combination EcoRⅠ/ TruⅠand EcoRⅠ/ MseⅠ

作　　者：徐文玲[1] 王淑芬[1] 牟晋华[1] 王翠花[1] 刘贤娴[1] 

机构地区：[1]山东省农业科学院蔬菜研究所,山东济南250100

出　　处：《山东农业科学》2008年第9期4-6,9,共4页Shandong Agricultural Sciences

基　　金：国家948项目(编号:2006-G13);山东省农业科学院青年科研基金(编号:2005YQ017)资助

摘　　要：通过对EcoRⅠ/TruⅠ与EcoRⅠ/MseⅠ两个双酶切组合的比较,证明在AFLP标记体系中,完全可以用内切酶TruⅠ替代昂贵的内切酶MseⅠ。EcoRⅠ与TruⅠ可同时加入,在不同温度下分段进行酶切:37℃酶切4 h,然后65℃酶切2 h,可达到分步酶切的效果。In this study, the effect of the restriction enzyme combination EcoR Ⅰ / Tru Ⅰ tor AFLF was compared with the combination EcoR Ⅰ / Mse Ⅰ. In this method, EcoR Ⅰ and Tru Ⅰ were added together with the digestion firstly at 37℃ for 4 hours, then at 65℃ for 2 hours. The results showed that Mse Ⅰ could be replaced by Tru I and the cost of AFLP could be reduced.

关 键 词：AFLP分子标记 内切酶 Tru Ⅰ MSE Ⅰ 

分 类 号：Q783.1[生物学—分子生物学]