香石竹环斑病毒的提纯及抗血清制备  

Purification and antiserum preparation of carnation ringspot virus.

作　　者：魏梅生[1] 相宁[1] 

机构地区：[1]农业部植物检疫实验所

出　　处：《植物检疫》1998年第1期8-10,共3页Plant Quarantine

摘　　要：选用豇豆黑种三尺作繁殖寄主，接种４周后采收病叶，采用聚乙二醇沉淀，差速离心和１０～４０％蔗糖梯度离心，得到提纯的香石竹环斑病毒，紫外吸收呈典型核蛋白吸收曲线，电镜下见到大量球状病毒粒体。采用多种途径免疫家兔获得的抗血清。Infected cowpea ( Vigna sesquipedalis) leaves were harvested 4 weeks after inoculation. The carnation ringspot virus was precipitated by adding polyethylene glycol. Further purify by repeated cycles of differential centrifugation and 10～40% sucrose density gradient centrifugation. The purified preparation had the ultraviolet absorption curve typical for nucleoprotein. Spherical particles were seen under electron microscopy. Rabbit given a series of intravenous or intramuscular injections yielded antiserum with titres 1∶16 in gel diffusion tests.

关 键 词：香石竹 环斑病毒 提纯 抗血清制备 

分 类 号：S432.41[农业科学—植物病理学] S436.8[农业科学—农业昆虫与害虫防治]