一种非开颅大鼠蛛网膜下腔出血动物模型的建立  

A NONCRANIOTOMY MODEL OF SUBARACHNOID HEMORRHAGE IN RATS

作　　者：金清东[1] 陈志[2] 刘智[2] 林秋泉[1] 胡胜利[2] 吴国材[2] John.H.Zhang 王宪荣[2] 冯华[2] 

机构地区：[1]福建医科大学莆田市第一医院教学医院神经外科,福建莆田351100 [2]第三军医大学西南医院神经外科,全军神经系统疾病微创诊治中心,重庆400038 [3]Division of Neurosurgery,Loma Linda University Medical Center

出　　处：《牡丹江医学院学报》2008年第6期5-8,共4页Journal of Mudanjiang Medical University

基　　金：国家自然科学基金资助项目(NO:30500662);莆田市科委资助项目(NO:2007S08)

摘　　要：目的:建立一种有效模拟临床颅内动脉瘤破裂后蛛网膜下腔出血病理生理过程的大鼠SAH模型。方法:显微操作经颈外动脉导入尼龙线至颈内动脉颅内段,刺破其分叉处造成大鼠SAH,观察各实验组大鼠死亡率、神经行为功能、脑含水量、大体病理解剖及海马区病理变化。结果:SAH模型成功率96.7%,死亡率10.34%;实验大鼠蛛网膜下腔发现血液或血凝块凝集;SAH后神经行为异常,24h神经功能评分为3.08±0.56分;脑含水量增加,皮层含水量为80.37±0.58%,脑干含水量为74.14±1.07%;海马CA1区神经元有水肿变性等改变。结论:非开颅血管内线穿刺法能可靠制备大鼠SAH模型并有效模拟临床颅内动脉瘤破裂出血后的蛛网膜下腔出血的病理生理过程。Objective：To establish a subarachnoid hemorrhage （SAH）model with simulated pathophysiologieal processes of clinic intracranial aneurysm rupture in rats. Methods：SAH was produced by passing a nylon thread through the right external carotid artery to internal carotid artery and piercing its furcation. Dynamic monitor of electroencephalogram and regional cerebral blood flow, the mortality and neurological deficits, brain water content, pathologic change were observed at 24h. Results：The achievement ratio of SAH model is 96. 7%, mortality 10. 34%. Neurological deficits was observed after SAH, mean neurological score 3.08 ± 0. 56. Brain water content increase and CA1 regions of hippocampus have hydropic degeneration at 24h after SAH. Conclusion：The noncraniotomy model of SAH in rats is reliable. It could effectively simulate clinic intracranial aneurysm rupture pathophysiological processes.

关 键 词：蛛网膜下腔出血 动物模型 大鼠 

分 类 号：R651[医药卫生—外科学]