乙型肝炎患者血清IL-12、IL-18、TGF-β_1、TIMP-1检测的临床意义  

Clinical significance of serum levels of IL-12, IL-18,TGF-β and TIMP-1 in patients with hepatitis B

作　　者：朱凤群[1] 霍康[1] 陈天艳[1] 李春姬[1] 王亚文[1] 赵英仁[1] 

机构地区：[1]西安交通大学医学院第一附属医院传染科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2008年第6期667-669,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省科学技术研究发展项目(No.2003K10-G62)

摘　　要：目的探讨血清IL-12、IL-18水平在乙型肝炎发生发展中的意义及与肝纤维化的关系。方法采用双抗体夹心ELISA法检测血清IL-12、IL-18、TGF-β1、TIMP-1水平。结果患者血清IL-12、IL-18、TGF-β1及TIMP-1水平与对照组相比均有显著性差异(P<0.05)。随慢性肝炎中度→慢性肝炎重度→肝硬化临床类型的逐渐加重,血清IL-18、TGF-β1、TIMP-1水平呈增高趋势,差别具有统计学意义(P<0.05)。TGF-β1、TIMP-1与PCⅢ、LN、IVC相比,相关系数r分别为0.986、0.998、0.925和0.921、0.987、0.814。结论乙型肝炎患者血清IL-12、IL-18、TGT-B1及TIMP-1水平和对照组相比有显著变化,且与病情变化有关。TGF-β1、TIMP-1与PCⅢ、LN、IVC有良好的相关性。Objective To investigate the significance of L-12 and IL-18 in serum of the progression of hepatitis B, the relation of the levels of TGF-β1, and TIMP-1 with liver cirrhosis, and explore the relation between the levels of these four cytokines. Methods The levels of IL-12, IL -18, TGF-β1 and TIMP-1 in serum were detected by ELISA. Results The levels of IL-12, IL-18, TGF-β1 and TIMP-1 differed significantly between hepatitis groups and the corresponding control groups （P〈0.05）. The level of IL-18, TIMP-1 and TGF-β1 increased obviously with the progression of the disease （P〈0.05）, from chronic hepatitis （moderate） to chonic hepatitis （severe） to cirrhosis （clinical type）. The correlational coefficient of TGF-β1 with PC Ⅲ, LN and IVC was 0. 986, 0. 998 and 0. 925, respectively; that of TIMP-1 with PCⅢ, LN and IVC was 0. 921, 0. 987 and 0. 814. Oonclusion The levels of IL-12, IL-18, TGF-β1 and TIMP-1 in serum have significant differences between hepatitis patients and the controls, and they are related with progression of the disease. TGF-β1 and TIMP-1 have significant correlation with PCⅢ, LN, and IVC.

关 键 词：乙型肝炎 肝纤维化 白细胞介素 

分 类 号：R512.6[医药卫生—内科学]