沙棘果油及沙棘籽油对大鼠放射性皮肤损伤的影响  

Effects of sea buckthorn pulp oil and sea buckthorn seed oil on radiation skin lesions in rats

作　　者：王养正[1,2] 吴英[1] Heikki Kallio Yang Baoru 王军宪[1] 王秉文[1] 崔刚[1] 

机构地区：[1]西安交通大学医学院药学系,陕西西安710061 [2]西安市第一医院眼病研究室,陕西西安710002 [3]芬兰土尔库大学生物化学及食品化学系,芬兰土尔库FIN-20014

出　　处：《西安交通大学学报（医学版）》2008年第6期705-709,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：与芬兰土尔库大学的科研协作项目,由芬兰土尔库大学提供资助(No.1996-08)

摘　　要：目的观察沙棘果油及沙棘籽油对大鼠放射性皮肤损伤的影响。方法实验用深部X线治疗机,每次以3000 cGy的剂量,照射两次,造成大白鼠臀部放射性皮肤损伤。待动物症状明显后,将Ⅱ度皮肤损伤的动物随机分为6组,即阴性对照组、沙棘果油高剂量组、沙棘果油低剂量组、沙棘籽油高剂量组、沙棘籽油低剂量组以及阳性对照组。沙棘果油及籽油高剂量组,每天在损伤部位涂药两次,而其低剂量组每天涂药一次,阴性对照组每天涂灭菌的注射用水一次,阳性对照组每天涂Solcoseryl-Jelly软膏一次。结果肉眼观察结果显示,连续用药10d后,沙棘果油、籽油及Sol-coseryl-Jelly组动物的局部红斑、水肿明显减轻,创面分泌物明显减少,用药2周后,上述症状明显消退,与阴性对照组比较,明显好转;另外,沙棘果油、籽油及Solcoseryl-Jelly组的创面愈合时间明显缩短。光学显微镜观察结果显示,沙棘果油、籽油及Solcoseryl-Jelly组的皮肤损伤明显减轻,炎症反应亦明显减轻,创面修复明显。电子显微镜观察结果显示,阴性对照组的超微结构明显受损,而沙棘果油、籽油及Solcoseryl-Jelly组的损伤明显减轻。结论沙棘果油及沙棘籽油对大鼠放射性皮肤损伤有明显的治疗作用。Objective To observe the effects of sea buckthorn pulp oil and sea buckthorn seed oil on radiation skin lesions of rats. Methods The experiment used deep x-ray therapeutic machine to irradiate the rats twice, and the irradiation dosage was 3 000 cGy each time. This caused radiation skin lesions to rats＇ buttock. After the symptoms became obvious, the rats suffering from Grade Ⅱ skin lesions were randomly divided into six groups： negative control group, high-dose sea buckthorn pulp oil group, low-dose sea buckthorn pulp oil group, high-dose sea buckthorn seed oil group, low-dose sea buckthorn seed oil group, and positive control group. The oil was applied to the wounded area twice a day for high-dose pulp oil group and high-dose seed oil group, and it was applied once a day for the low-dose groups. For the negative control group, sterilized injection water was applied once a day, and Solcoseryl-Jelly ointment was applied once a day for the positive control group. Results The results from naked eye observation showed that after medication for 10 consecutive days, the local erythema and edema of the groups using sea buckthorn pulp oil and seed oil and Solcoseryl-Jelly ointment abated, and the secretions of the Wounded surface reduced considerably. After medication for two weeks, obvious disappearance of the above symptoms was observed; compared with the negative control group, the condition improved considerably. In addition, the healing time of the wounded area of the rats was markedly shortened for the pulp oil groups, seed oil groups, and the Solcoseryl-Jelly group. Optical microscopic observation showed that the injuries in the pulp oil group,seed oil group, and the Solcoseryl-Jelly group were significantly reduced, inflammatory reaction was obviously reduced, and the restoration of the wounded areas was obvious. Electron microscopic observation showed that the ultrastrueture of the negative group was obviously damaged, but that of the pulp oil groups, seed oil groups, and Solcoseryl-Jelly group reduced s

关 键 词：沙棘果油 沙棘籽油 放射性皮肤损伤 大鼠 

分 类 号：R961[医药卫生—药理学]