动脉粥样硬化对大鼠心肌钙敏感受体表达和细胞凋亡的影响  

Increased myocardial expression of calcium-sensing receptor and apoptosis in a rat model of atherosclerosis

作　　者：郭津[1] 徐长庆[1] 李鸿珠[1] 王丽娜[1] 王鲁川[2] 张力[1] 张伟华[1] 李光伟[1] 田野[1] 

机构地区：[1]哈尔滨医科大学病理生理学教研室,150086 [2]佳木斯大学第一附属医院骨一科

出　　处：《中华心血管病杂志》2008年第12期1101-1105,共5页Chinese Journal of Cardiology

基　　金：国家自然科学基金资助项目（30370577,30700288）;教育部高校博士点基金资助项目（20070226012）;黑龙江省研究生创新科研资金资助项目（YJSCX2008-106HLJ）;哈尔滨医科大学研究生创新基金资助项目（HCXB2008006）

摘　　要：目的观察高脂血症和动脉粥样硬化对大鼠心肌钙敏感受体（CaSR）表达和细胞凋亡的影响。方法采用腹腔注射维生素（Vit）D3（6×10^5U／kg）＋高脂饮食6周的方法，建立大鼠高脂血症和动脉粥样硬化模型。Wistar大鼠随机分为正常对照组（n=12）和动脉粥样硬化组（n=12）。采用RT—PCR和Westernblot分别观察CaSR、Bax、Bcl-2、caspase-3的mRNA和蛋白表达。TUNEL染色观察心肌细胞凋亡情况。光镜观察腹主动脉和心肌形态学变化。电镜观察心脏超微结构变化。紫外分光法检测乳酸脱氢酶、肌酸激酶、超氧化物岐化酶的活性和丙二醛的含量，电化学免疫发光法检测肌钙蛋白水平。结果动脉粥样硬化组乳酸脱氢酶和肌酸激酶活性、丙二醛含量和肌钙蛋白水平、细胞凋亡指数以及CaSR、Bax和caspase-3的表达均高于对照组，而超氧化物岐化酶活性则低于对照组，Bcl-2表达低于对照组，心肌细胞超微结构损伤严重。结论高脂血症和动脉粥样硬化可引起大鼠心肌CaSR的表达增加和细胞凋亡，其机制与心肌缺血所致的氧化应激有关。Objective To observe the effect of hyperlipidemia and atherosclerosis on rat myocardial expression of calcium-sensing receptor and apoptosis. Methods The rat atherosclerosis model was induced by intraperitoneal injection of VD3 （6 × 10^5 U/kg） and high cholesterol diet. Wistar rats were divided into two groups： （1） Control group; （2） AS group（n = 12 each）. The expressions of CaSR, Bcl-2, Bax and easpase-3 were analyzed by Western blot and RT-PCR. Apoptotie cells were observed by TUNEL assay. The morphological changes of abdominal aorta and cardiac tissues were observed under optical and eleetro microscopes. The activity of LDH, CK, SOD and the content of MDA were assayed with ultraviolet spectrophotometer. The level of cTnT was detected by electrochemical immunofluoresence. Results Compared with control group, the activity of LDH and CK, the content of MDA and cTnT, the apoptosis index, the expression of CaSR, Bax and easpase-3 were significantly increased, but the SOD activity and Bcl-2 expression were significantly decreased, the myocardial uhrastructure injury was significantly aggravated in the AS group （ all P 〈 0. 05 ）. Conclusion Hyperlipidemia and atherosclerosis can up-regnlate myocardial calcium-sensing receptor expression, promote myocardial apoptosis, aggravate oxidative stress and myocardial ischemia.

关 键 词：动脉粥样硬化 受体 钙敏感 心肌 细胞凋亡 

分 类 号：R686[医药卫生—骨科学]