大豆内源基因调控与籽粒不饱和脂肪酸的形成  

Endogenous Gene Regulate the Formation of Unsaturated Fatty Acid of Soybean

作　　者：李丽丽[1,2] 张雷[1] 刘丽君[1] 唐晓飞[1] 杨喆[1] 

机构地区：[1]黑龙江省农业科学院大豆研究所,黑龙江哈尔滨150086 [2]东北农业大学研究生学院,黑龙江哈尔滨150030

出　　处：《大豆科学》2008年第6期929-932,共4页Soybean Science

基　　金：国家高技术研究发展计划(863计划)资助项目(2006AA1021F9);大豆产业技术体系资助项目

摘　　要：大豆亚油酸主要的合成途径是在大豆内源基因fad2调控下由油酸生成亚油酸。与此同时,在内源基因fad3作用下,亚油酸又部分转化成为亚麻酸。采用7个国内外具有一定代表性的栽培品种,对大豆籽粒成熟过程中不同时期的样本进行定量PCR分析。结果表明:不饱和脂肪酸的合成过程是一个连锁反应,单独一个基因的表达不但影响与其相关的代谢途径,而且连带影响其他脂肪酸成分的积累。fad3的高量表达,促进不饱和脂肪酸积累总量的增加。籽粒发育后期,油酸含量下降,亚油酸,亚麻酸含量稳步上升。当fad2表达受抑制时,亚油酸含量也较低。Linoleie acid is one of the helpful composition in soybean for human health. Its main synthesis approach is from oleic acid under the regulation of endogenous gene fad2. At the same time, endogenous gene fad3 plays some effect on the synthesis of linoleic acid. Seven soybean cultivars （lines）were planted and soybean pods were sampled every 5 days from full flowering,and the dynamic accumulation of unsaturated fatty acid and expression of fad2 and fad3 gene in soybean seeds were determined. Result suggested that the synthesis of unsaturated fatty acids was a chain reaction,the expression of single gene influenced both its related metabolic pathway and the accumulation of other unsaturated fatty acid. The high expression of fad3 promoted the whole synthesis process. During the final stage of seed development ,oleic acid content decreased whlie linoleic and linolenie content increased steadily. The linoleic content was lower when the expression of fad2 was inhibited.

关 键 词：大豆 亚油酸 荧光定量 FAD2 fad3 

分 类 号：S565.1[农业科学—作物学]