辐射损伤后大鼠鼻黏膜早期组织重塑的特点  

Early remodeling of nasal mucosa in rat model after radiation injury

作　　者：肖芒[1] 汤建国[1] 施国治[2] 罗宝珍[1] 赵丽娜[1] 

机构地区：[1]浙江大学医学院附属邵逸夫医院耳鼻咽喉-头颈外科浙江省生物治疗重点实验室,杭州310016 [2]浙江大学医学院附属邵逸夫医院放疗科,杭州310016

出　　处：《中华放射医学与防护杂志》2008年第6期602-605,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：浙江省自然科学基金资助项目（Y205073）

摘　　要：目的探讨辐射损伤后大鼠鼻黏膜早期组织重塑的特点。方法清洁级SD大鼠40只，雄性，采用随机数字排列法将动物随机分组，对照组、20Gy组、30Gy组、40Gy组和50Gy组，每组8只。辐射完成后2周，取出中鼻甲进行组织学处理：采用苏木素-伊红（hematoxylin-eosin，HE）染色，计数中鼻甲黏膜内嗜酸粒细胞数并测定上皮细胞损伤程度；采用阿辛蓝-过碘酸-希夫（alcian blueperiodic acid-Schif，AB-PAS）染色，计数中鼻甲黏膜中单位面积上皮细胞中杯状细胞数；采用Masson三色胶原染色Masson trichrome（MT）法染色，测定中鼻甲黏膜内胶原成分染色的百分比面积。结果对照组少量嗜酸粒细胞浸润，实验组均有大量嗜酸粒细胞浸润，并以30Gy组嗜酸性粒细胞浸润最多。20Gy组未见明显的上皮细胞损伤，其他各组均见不同程度的上皮细胞损伤，吸收剂量越大，上皮细胞损伤程度越重。20Gy组杯状细胞数略增多，但差异无统计学意义；30Gy组和40Gy组杯状细胞明显增多，50Gy组杯状细胞数明显减少。细胞外基质沉积在20Gy组略增多，但差异无统计学意义；其他各组明显增多，且随着剂量的增大，MT染色蓝色所占的百分比面积有增多的趋势。结论黏膜上皮细胞损伤、嗜酸性粒细胞浸润、杯状细胞化生和黏膜内胶原成分增加是早期放射性鼻黏膜损伤后黏膜上皮组织重塑的特点。组织重塑随着辐射剂量和辐射时间呈现一定的变化规律。Objective To explore the feature of nasal mucosa remodeling in experimental radiation injury. Methods Fourty male rats were randomly divided into five groups, as control group and radiation injury groups （radiation dose were 20 Gy,30 Gy,40 Gy and 50 Gy）. Each group had 8 rats. Two weeks after the last irradiation, the rats were killed and the nasal middle turbinates of the animals were removed. The tissue blocks were embedded in paraffin. The paraffin sections were stained with hematoxylin and eosin （ HE）, aleian blueperiodie aeid-Sehif （AB-PAS）, and Masson Triehrome （MT）. The infiltrating eosinophils in nasal mucosa were examined. AB-PAS positive cells in the surface epithelium in nasal mueosa were counted. The percentage of area in MT stained extraeellular matrix in nasal mueosa and damage of epithelium were determined by an image analyzer. Results The control group only presented a few eosinophils. Significant eosinophil infiltration was observed in the radiation injury groups, especially for the 30 Gy radiation injury group. Compared with the control group, there was no significant epithelial damage in 20 Gy radiation injury group. Significant epithelial damage were observed in the rest of radiation injury groups. The epithelial damage became more severe as the radiation dose increasing. A little but not significant increase in AB-PAS positive cells was observed in the mueos of the 20 Gy radiation injury group and significant increase in the 30 and 40 Gy groups. But in the 50 Gy radiation injury group, the AB-PAS positive cells were decreased compared with control group. The collagen fibrils in the mucosa of nasal middle turbinate in 20 Gy radiation injury group did not significantly increase. But in the other groups, the increase was significant compared with that of control group. Furthermore, collagen fibrils increased as the radiation dose increased. Conclusions Epithelial damage, goblet cells hyperplasia and extraeellular matrix deposition are the features of remodeling in the rats model

关 键 词：辐射损伤 动物模型 上皮细胞 杯状细胞 细胞外基质 

分 类 号：R144[医药卫生—公共卫生与预防医学]